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目的:检测妊娠期肝内胆汁淤积症(ICP)产妇脂质过氧化物及雌激素水平改变,借以探讨其相关的临床意义。方法:收集该院产科2008年12月～2012年12月住院分娩的妊娠期肝内胆汁淤积症64例,另取该院同期分娩的健康产妇40例作为对照,ICP孕妇分为轻度组(n=33)和重度组(n=31)。超氧化物歧化酶(SOD)和丙二醛(MDA)的检测采用分光分析法,雌二醇(E2)测定使用ELISA法。结果:对照组中SOD、MDA和E2表达水平分别为(204.41±26.57)U/ml、(16.43±4.22)nmol/ml和(157.15±26.44)μg/ml,ICP组中分别为(212.37±27.41)U/ml、(34.63±7.15)nmol/ml和(232.72±36.52)μg/ml,统计分析显示SOD在两组之间不具有显著性差异(P>0.05),而MDA和E2在ICP组显著高于对照组(P<0.01)。轻度组中SOD、MDA和E2表达水平分别为(211.26±25.31)U/ml、(28.54±5.03)nmol/ml和(201.62±30.73)μg/ml,重度组中分别为(213.72±28.24)U/ml、(41.11.63±7.84)nmol/ml和(265.83±41.84)μg/ml,统计分析显示SOD在两组之间不具有统计学差异(P>0.05),而MDA和E2在轻度组显著低于重度组(P<0.05)。结论:妊娠期肝内胆汁淤积症产妇体内存在MDA和E2的异常高表达,高的脂质过氧化状态和雌激素水平可能参与了妊娠肝内胆汁淤积症的发生、发展过程。
Objective: To detect changes of lipid peroxidation and estrogen levels in pregnant women with intrahepatic cholestasis of pregnancy (ICP), in order to explore its clinical significance. Methods: 64 cases of intrahepatic cholestasis of pregnancy during hospital delivery from December 2008 to December 2012 in our hospital were collected. In addition, 40 healthy pregnant women delivered at the same period of the hospital were used as control. ICP pregnant women were divided into mild group n = 33) and severe group (n = 31). Superoxide dismutase (SOD) and malondialdehyde (MDA) were detected by spectrophotometry and estradiol (E2) by ELISA. RESULTS: The levels of SOD, MDA and E2 in the control group were (204.41 ± 26.57) U / ml, (16.43 ± 4.22) nmol / ml and (157.15 ± 26.44) μg / ml, respectively, and were 212.37 ± 27.41 (34.63 ± 7.15) nmol / ml and (232.72 ± 36.52) μg / ml, respectively. Statistical analysis showed that there was no significant difference in SOD between the two groups (P> 0.05) Significantly higher than the control group (P <0.01). The levels of SOD, MDA and E2 were (211.26 ± 25.31) U / ml, (28.54 ± 5.03) nmol / ml and (201.62 ± 30.73) μg / ml respectively in the mild group and (213.72 ± 28.24) U / ml, (41.11.63 ± 7.84) nmol / ml and (265.83 ± 41.84) μg / ml, respectively. Statistical analysis showed that there was no significant difference in SOD between the two groups (P> 0.05) Degree group was significantly lower than the severe group (P <0.05). Conclusion: The expression of MDA and E2 in pregnant women with intrahepatic cholestasis of pregnancy is abnormally high. The high lipid peroxidation status and estrogen level may be involved in the occurrence and development of intrahepatic cholestasis of pregnancy.