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目的:通过改进成膜工艺,减少血液中HCO3-对海藻酸钠-ε-聚赖氨酸(ε-AP)微胶囊稳定性的影响,从而增强ε-AP微胶囊在血液中的稳定性,使其能够应用于生物人工肝血浆/血液灌注。方法:改进成膜工艺:在成膜液中添加10 mmol/L的Na HCO3并增加ε-聚赖氨酸反应浓度来制备ε-AP微胶囊,用膨胀度表征其机械强度,荧光标记的牛血清白蛋白(FITC-BSA)检测其通透性能,并以微胶囊的膨胀度变化作为衡量指标,考察微胶囊在Na HCO3溶液和血清中的稳定性。结果:改进成膜工艺制备的ε-AP微胶囊与常规条件制备的微胶囊具有接近的机械强度和通透性;改进成膜工艺能够增强的ε-AP微胶囊在Na HCO3溶液和血清中的稳定性;常规条件制备的ε-AP微胶囊在血清中膨胀度增加了约560%,而改进成膜工艺制备的微胶囊膨胀度仅增加160%,其稳定性显著增强。结论:改进成膜工艺能够增强ε-AP微胶囊在血液中的稳定性。
OBJECTIVE: To improve the stability of ε-AP microcapsules in the blood by improving the film-forming process and reducing the influence of HCO3- in the blood on the stability of sodium alginate-ε-polylysine (ε-AP) microcapsules, It can be applied to bioartificial liver / blood perfusion. Methods: The film-forming process was improved. Ε-AP microcapsules were prepared by adding 10 mmol / L Na HCO3 and increasing the reaction concentration of ε-polylysine. The mechanical strength, fluorescence intensity The permeability of serum albumin (FITC-BSA) was measured. The swelling degree of microcapsules was used as a measure to evaluate the stability of microcapsules in Na HCO3 solution and serum. Results: The ε-AP microcapsules prepared by the improved film-forming process have close mechanical strength and permeability to the microcapsules prepared under the conventional conditions; the ε-AP microcapsules with improved film forming process can be improved in Na HCO3 solution and serum Stability. The ε-AP microcapsule prepared in the conventional condition increased about 560% in the serum, while the microcapsule prepared by the improved film-forming process increased only by 160%, and its stability was remarkably enhanced. Conclusion: Improving the film-forming process can enhance the stability of ε-AP microcapsules in the blood.