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运用MSAP技术测定水稻(Oryza sativa L.)纯系品种‘日本腈’和‘松前’经萘染毒胁迫后,不同生长时期叶片基因组DNA甲基化变异的情况.结果表明,水稻经萘胁迫后存在基于DNA甲基化水平和模式改变的表观遗传变异;5-甲基胞嘧啶百分含量的变化无统一趋势或规律;全部检测到的1051个位点中,日本腈有16.56%发生了变异,相对于松前的12.08%具有显著差异,一定程度上说明抗萘胁迫能力与基因型有关:松前强于日本腈;不同基因型及不同生长时期DNA甲基化模式的变异存在明显差异,表现为以去甲基化为主(0.48%~10.41%),超甲基化较少(0.10%~1.92%),由此推测DNA去甲基化可能是植物抗萘胁迫机制的一部分.
MSAP was used to determine the variation of genomic DNA methylation in leaves of pure rice varieties (Oryza sativa L.) at different growth stages after being exposed to Naphthalene stress. The results showed that after NAP There was an epigenetic variation based on DNA methylation level and pattern change; there was no uniform trend or regularity in the percentage change of 5-methylcytosine; of all the 1051 loci detected, 16.56% of nitrile occurred in Japan The variation was significantly different from that at 12.08%. To some extent, the ability of resisting naphthalene stress was related to genotypes: pine was stronger than that of Japanese nitrile. There were significant differences in DNA methylation patterns among different genotypes and growth periods To demethylation (0.48% ~ 10.41%), less hypermethylation (0.10% ~ 1.92%), suggesting that DNA demethylation may be part of the plant anti-naphthalene stress mechanism.