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以2份耐盐大麦和3份未经耐盐性鉴定的大麦为供试材料,比较了它们的离体培养花药对诱导培养基中添加0.3%NACL、愈伤组织对分化培养基中添加0.3%NACL的培养反应。实验结果表明:在不添加NACL诱导培养基上培养的花药反应率与供试材料耐盐性无相关性;不添加NACL培养基上形成的愈伤组织在不添加NACL分化培养基上形成绿苗分化率与供试材料的耐盐性相关性不明显;添加NACL培养基上形成的花药反应率与供试材料耐盐性呈明显相关;添加NACL培养基上形成的绿苗分化率与供试材料的耐盐性相关性明显;愈伤组织诱导和苗分化二阶段连续给予NACL胁迫比分别给予NACL胁迫更易鉴定出供试材料的耐盐性。
Two salt-tolerant barley cultivars and three barley cultivars without salt tolerance were used as experimental materials. Compared with their anther culture in vitro, 0.3% NACL was added to the induction medium and 0.3 % NACL culture reaction. The results showed that there was no correlation between anther response rate and salt tolerance of NACL induction medium. No callus formed on NACL medium was formed into green seedlings without NACL differentiation medium There was no significant correlation between the differentiation rate and the salt tolerance of the tested materials. The anther reaction rate formed on the NACL medium was significantly correlated with the salt tolerance of the tested materials. The salt tolerance of the materials was obvious. NACL stress induced by two stages of callus induction and seedling differentiation was more easily identified than the NACL stress salt tolerance of the tested materials.