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目的：建立测定人血浆中阿那曲唑药物浓度的ＧＣ－ＥＣＤ方法。方法：以安定为内标，用乙醚一次提取，ＨＰ－５０毛细管柱测定。结果：检测浓度在０．５－２０μｇ·Ｌ－１内，线性方程为Ｙ＝０．０１７８ｃ－００２８４，ｒ＝０．９９９８。方法回收率平均为９７．５４％，３种浓度１．０，１０．０，２０．０μｇ·Ｌ－１提取回收率分别为８４．５４％，９３．９４％，９７．０３％；日内、日间精密度分别为４．７９％，２．１３％，１．７１％及６．７０％，２．９６％，４．１７％（ｎ＝５），在志愿者单剂量口服阿那曲唑１ｍｇ后，０．３３－１０６ｈ内血药浓度范围在１．２７－１０．１９μｇ·Ｌ－１。结论：该法简便、准确、灵敏，可用于测定该药在人血浆中的药物浓度。
Objective: To establish a GC-ECD method for the determination of anastrozole in human plasma. Methods: Diazepam was used as internal standard and extracted once with ether and determined by HP-50 capillary column. Results: The linear equation was Y = 0.0178c-00284 and r = 0.9998 at 0.5-20μg · L-1. The recovery of the method was 97.54% on average, and the recoveries of the three concentrations of 1.0, 10.0, 20.0 μg · L-1 were 84.54%, 93.94% and 97.03%, respectively. The intra-day precision was 4.79%, 2.13%, 1.71% and 6.70%, 2.96% and 4.17% respectively (n = 5) After 1mg, 0.33-106h blood concentration within the range of 1.27-10.19μg · L-1. Conclusion: The method is simple, accurate and sensitive and can be used to determine the drug concentration in human plasma.