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目的研究CCR5△32、CCR5m303、CCR264I、SDF13′A基因多态性对中国HIV1感染者预后的影响。方法对在深圳地区发现的HIV1感染者进行流行病学调查,应用PCR/RFLP技术分析感染者CCR5△32、CCR5m303、CCR264I、SDF13′A4种基因的多态性,对部分人群进行HIV1血浆病毒载量和CD4+细胞计数的检测,判断感染者的潜伏期,使用SPSS11.0统计软件分析基因多态性对感染者病毒载量和潜伏期的影响。结果在189例HIV1感染者中没有发现CCR5△32和CCR5m303突变基因型,SDF13′A的等位基因频率为26.14%,CCR264I的等位基因频率为19.82%。方差分析发现,CCR264I基因野生型与杂合型的两组HIV1感染者人群病毒载量对数的大小差异无统计学意义(P=0.272),两组人群潜伏期的长短差异无统计学意义(P=0.662)。SDF1基因野生型、杂合型和纯合型的3组HIV1感染者人群病毒载量对数的大小差异有统计学意义(P=0.001),但3组人群潜伏期的长短差异无统计学意义(P=0.228)。结论CCR264I基因突变对中国汉族HIV1感染者病毒载量没有明显影响,因而也不影响感染者的潜伏期。SDF13′A基因突变对于病毒载量有降低作用,但对延长感染者潜伏期可能没有作用。
Objective To investigate the effects of CCR5 △ 32, CCR5m303, CCR264I and SDF13’A gene polymorphisms on the prognosis of HIV1 in China. Methods Epidemiological investigation of HIV1 infected persons found in Shenzhen area was carried out. The polymorphisms of CCR5 △ 32, CCR5m303, CCR264I and SDF13’A genes were analyzed by PCR / RFLP and the prevalence of HIV1 plasma viral load And CD4 + cell count to determine the incubation period of the infected patients. SPSS11.0 statistical software was used to analyze the effect of genetic polymorphisms on the viral load and latency of infected persons. Results No genotypes of CCR5 △ 32 and CCR5m303 were found in 189 HIV1 infected persons. The allele frequency of SDF13’A was 26.14% and the allele frequency of CCR264I was 19.82%. Variance analysis showed that there was no significant difference in the logarithm of viral load between the two groups of HIV1-infected and wild-type CCR264I (P = 0.272). There was no significant difference in latency between the two groups (P = 0.662). There were significant differences in the logarithm of viral load between the three groups of HIV-1 infected SDF1 gene wild-type, heterozygous and homozygous groups (P = 0.001), but there was no significant difference in latency between the three groups P = 0.228). Conclusion The mutation of CCR264I has no obvious influence on the viral load of HIV1 in Chinese Han, and therefore does not affect the incubation period of infected persons. SDF13’A gene mutation has a reduced effect on viral load, but may have no effect on prolonging the incubation period of the infected person.