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目的:探讨T淋巴瘤侵袭转移诱导因子1(T-lymphoma invasion and metastasisi nducing proteinI,Tiam1)在早孕小鼠子宫内膜中的表达规律。方法:采用RT-PCR和Western blotting方法分别检测早孕小鼠子宫内膜中Tiam1mRNA和蛋白的表达水平。结果:①Tiam1mRNA的表达量随妊娠天数的增加逐渐增高,到妊娠第5日表达量达到峰值,于妊娠第6日、第7日开始逐渐降低;②Tiam1蛋白表达与其mRNA表达规律基本一致。结论:Tiam1在妊娠早期子宫内膜中持续表达,可能参与小鼠胚胎植入过程。
Objective: To investigate the expression of T-lymphoma invasion and metastasis in wound endometrium of early pregnant mice. Methods: The expression of Tiam1 mRNA and protein in endometrium of early pregnancy mice were detected by RT-PCR and Western blotting respectively. Results: (1) The expression of Tiam1 mRNA gradually increased with the increase of the number of days of pregnancy, reaching its peak on the 5th day of pregnancy, and gradually decreased on the 6th day and the 7th day of pregnancy; (2) The expression of Tiam1 was basically the same as the mRNA expression. Conclusion: Tiam1 is expressed continuously in the early stage of pregnancy and may be involved in the process of mouse embryo implantation.