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目的:探讨Genistein增加顺铂诱导的耐药卵巢癌细胞SKOV-3凋亡的可能作用机制。方法:倒置相差显微镜下观察药物处理后细胞形态学的变化;MTT比色法检测不同药物处理后对SKOV-3细胞增殖的影响;流式细胞仪检测药物处理后细胞的凋亡情况;流式细胞仪和荧光显微镜检测细胞内活性氧(ROS)的水平。结果:10ug/ml的Genistein和2.5ug/ml的顺铂联用24h后,引起了细胞内ROS的增加,细胞的凋亡率也显著增高,与单用顺铂组相比差异有显著性(P<0.05);用NAC预处理细胞2h后,有效抑制了ROS的产生,并增加了细胞的活性,降低了细胞的凋亡率,与未加NAC组相比差异有显著性(P<0.05)。结论:Genistein增加顺铂诱导的耐药卵巢癌细胞SKOV-3的凋亡与细胞内ROS水平的升高有关,这可能是Genistein增加顺铂诱导的耐药卵巢癌细胞SKOV-3凋亡的作用机制之一。
Aims: To investigate the possible mechanism of Genistein increasing cisplatin-induced apoptosis of ovarian cancer SKOV-3 cells. Methods: The morphological changes of the cells were observed under inverted phase contrast microscope. The effects of different drugs on the proliferation of SKOV-3 cells were detected by MTT colorimetric assay. The apoptosis of SKOV-3 cells was detected by flow cytometry. Cytometry and fluorescence microscopy were used to detect intracellular reactive oxygen species (ROS) levels. Results: Genistein at 10 ug / ml and cisplatin at 2.5 ug / ml for 24 h resulted in an increase of intracellular ROS and a marked increase of apoptosis rate, which was significantly different from that of cisplatin alone P <0.05). Pretreatment of cells with NAC for 2 h effectively inhibited the production of ROS, increased the activity of cells and decreased the apoptosis rate of cells, which was significantly different from that of NAC group (P <0.05) ). CONCLUSION: Genistein can increase the apoptosis of SKOV-3 cells induced by cisplatin, which may be related to the increase of cisplatin-induced apoptosis of ovarian cancer SKOV-3 cells. One of the mechanisms.