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[目的]探讨核糖体S6蛋白激酶4(ribosomal protein S6 kinase 4 gene,RSK4)基因对乳腺癌细胞Ki-67、cyclin D1、CXCR4、HIF-1α、E-cadherin基因转录影响。[方法]分别构建RSK4干扰载体、过表达载体转染至乳腺癌MCF-7细胞及MDA-MB-231细胞中,RT-PCR比较实验组和对照组Ki-67、cyclin D1、CXCR4、HIF-1α及E-cadherin mRNA表达水平。[结果]干扰组CXCR4、HIF-1α、cyclin D1及Ki-67mRNA的转录水平分别为2.238±0.0711、1.672±0.0833、1.540±0.0115、1.390±0.0902,与空白对照组、阴性对照组相比均升高,但E-cadherin mRNA的转录水平明显降低,差异有统计学意义(P<0.05)。过表达组Ki-67、cy-clinD1、CXCR4和HIF-1α转录水平分别是0.603±0.0066、0.460±0.0155、0.424±0.0198、0.205±0.0082,与空白对照组、阴性对照组相比均降低,而E-cadherin mRNA转录水平升高,差异有统计学意义(P<0.05)。[结论]RSK4基因表达影响乳腺癌细胞增殖黏附相关基因Ki-67、cyclinD1、CXCR4、HIF-1α和E-cadherin mRNA转录水平。
[Objective] To investigate the effect of ribosomal protein S6 kinase 4 (RSK4) gene on the transcription of Ki-67, cyclin D1, CXCR4, HIF-1α and E-cadherin in breast cancer cells. The expression of Ki-67, cyclin D1, CXCR4 and HIF-1α in breast cancer cell line MCF-7 and MDA-MB-231 cells were detected by RT- 1α and E-cadherin mRNA expression levels. [Results] The transcriptional levels of CXCR4, HIF-1α, cyclin D1 and Ki-67 mRNA in the interference group were 2.238 ± 0.0711, 1.672 ± 0.0833, 1.540 ± 0.0115 and 1.390 ± 0.0902 respectively, both increased compared with the blank control group and the negative control group High, but the transcription level of E-cadherin mRNA was significantly lower, the difference was statistically significant (P <0.05). The transcription levels of Ki-67, cy-clinD1, CXCR4 and HIF-1α in overexpression group were 0.603 ± 0.0066, 0.460 ± 0.0155, 0.424 ± 0.0198 and 0.205 ± 0.0082, respectively, which were lower than those in blank control group and negative control group E-cadherin mRNA transcription level increased, the difference was statistically significant (P <0.05). [Conclusion] The expression of RSK4 gene affects the mRNA transcription of Ki-67, cyclinD1, CXCR4, HIF-1α and E-cadherin in breast cancer cells.