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目的探讨CalyculinA诱导早熟染色体凝集技术作为低剂量电离辐射生物剂量计的可行性。方法取健康成年人的抗凝外周静脉血,用X射线分别以0、0.1、0.25、0.5、0.75和1.0Gy照射。照射后将血样接种于RP-MI1640培养基中,于37℃、5%CO2恒温培养箱内培养48h,当培养24h时加入秋水仙素,终止培养前2h加入Calyculi-nA诱导早熟凝集染色体,收获制片并用Giemsa染液染色,观察G1、G2/M-PCC细胞中总畸变率、断片率、双着丝粒体加着丝粒环率与辐射剂量的效应关系。结果用CalyculinA可成功诱导人外周血淋巴细胞产生PCC,且总畸变率、断片率、双着丝粒体加着丝粒环率随照射剂量的增加而增加,最佳拟合曲线为二次多项式模式。结论 CalyculinA诱导PCC技术可以作为一种方法来估算低剂量受照者的辐射剂量,但是需要更多的资料来完善。
Objective To investigate the feasibility of using Calyculin A to induce precocious chromosome agglutination as a bioassay dosimeter for low dose ionizing radiation. Methods Peripheral anticoagulant blood was collected from healthy adults and irradiated with X-rays at 0, 0.1, 0.25, 0.5, 0.75 and 1.0 Gy, respectively. After irradiation, the blood samples were inoculated into RP-MI1640 medium and cultured at 37 ° C in a 5% CO2 incubator for 48h. When cultured for 24 hours, colchicine was added, and Calyculi-nA was added 2h prior to termination of culture to induce premature aggregation of the chromosomes. The cells were stained and stained with Giemsa stain to observe the effect of radiation dose on the total aberration rate, fragment rate, ciliary body centromeric rate and centromeric ring rate in G1 and G2 / M-PCC cells. Results CalyculinA could induce PCC production in human peripheral blood lymphocytes, and the total aberration rate, fragment rate, dicentricity and centromere ring rate increased with the increase of irradiation dosage. The best fitting curve was quadratic polynomial mode. Conclusions The Calyculin A-induced PCC technique can be used as a method to estimate the radiation dose of low-dose irradiated subjects, but more information is needed to improve it.