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目的:研究灯盏花素对正畸牙移动大鼠牙槽骨重建的影响。方法:56只雄性SD大鼠随机分为对照组和实验组,每组28只,建立大鼠正畸牙移动模型,50g力正畸装置牵引上颌第一磨牙近中移动,隔日实验组每日灌服灯盏花素100㎎/㎏,对照组每日给予同等剂量生理盐水。两组大鼠分别在加力第7、14、21、28天后分批处死,测量各个时间点上颌第一磨牙的移动距离,HE染色法观察牙周组织及压力侧破骨细胞数量变化,免疫组织化学染色法观察牙槽骨中压力侧IL-1β表达变化。结果:正畸加力第7天实验组(灯盏花素100㎎/㎏)大鼠牙移动距离与对照组无显著性差异,破骨细胞数量增加有显著性差异,牙槽骨中IL-1β阳性表达增强,与对照组有显著性差异。加力第14、21、28天实验组大鼠牙移动距离比对照组分别增加0.12mm、0.23mm、0.19mm,破骨细胞数量比对照组分别增加1.27、1.49、1.56,两组比较差异有显著性,IL-1β表达平均灰度比对照组分别增加34.37、33.94、30.91,两组比较差异有显著性。结论:灯盏花素通过上调牙周组织中IL-1β的表达,促进破骨细胞的增殖和分化,有利于正畸牙移动。
Objective: To study the effect of breviscapine on alveolar bone remodeling after orthodontic tooth movement in rats. Methods: Fifty-six male Sprague-Dawley rats were randomly divided into control group and experimental group, with 28 rats in each group. The model of orthodontic tooth movement was established in rats. 50g force orthodontic device was used to pull the maxillary first molars close to each other. Breviscapine 100 ㎎ / ㎏, the control group given the same dose of saline daily. The rats in each group were sacrificed on days 7, 14, 21 and 28, respectively. The movement distance of maxillary first molar at each time point was measured. The number of periodontal tissue and pressure-side osteoclast were observed by HE staining. Histochemical staining was used to observe the expression of IL-1β in the pressure side of alveolar bone. RESULTS: There was no significant difference in tooth movement distance between the experimental group (breviscapine 100 ㎎ / ㎏) and the control group on the 7th day after orthodontic force. There was a significant difference in the number of osteoclasts. The level of IL-1β Positive expression increased, and the control group were significantly different. On the 14th, 21st and 28th days, the tooth movement distance in the experimental group increased by 0.12mm, 0.23mm and 0.19mm respectively compared with the control group, and the number of osteoclasts increased by 1.27, 1.49 and 1.56 respectively compared with the control group Significantly, the average gray level of IL-1β expression increased by 34.37, 33.94 and 30.91 respectively compared with the control group, the difference was significant between the two groups. Conclusion: Breviscapine can promote the proliferation and differentiation of osteoclasts by up-regulating the expression of IL-1β in periodontal tissues, which is in favor of orthodontic tooth movement.