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为探讨婴儿肝炎综合征（ＮＨＳ）与疱疹病毒（ＨＳＶ）感染的关系。在疱疹病毒高度同源序列ＤＮＡ聚合酶基因中设计一对引物，能对ＨＳＶ—Ⅰ、ＨＳＶ—Ⅱ、ＥＢＶ和ＣＭＶ四种疱疹病毒ＤＮＡ进行扩增，继而用凝胶电泳和限制性内切酶ＢａｍＨⅠ或ＳｍａⅠ酶切分析扩增产物进行鉴别，建立了能一次性分型检测上述四种病毒的ＰＣＲ—酶谱法。灵敏度可测到１ｆｇＤＮＡ，且该引物仅对四种疱疹病毒扩增。用建立的ＰＣＲ—酶谱法检测３０例ＮＨＳ的患儿尿中ＣＭＶ、ＨＳＶ—Ⅰ、ＨＳＶ—Ⅱ、ＥＢＶ阳性率分别为６６．６７％、３０％、３．３３％、６．６７％。１２例同日龄肺炎治愈新生儿ＣＭＶ阳性检出率为１６．７％，与ＮＨＳ的ＣＭＶ感染率差异显著，Ｐ＜０．０５。而ＨＳＶ—Ⅰ、ＨＳＶ—Ⅱ、ＥＢＶ的感染率在ＮＨＳ与正常儿无明显差异。提示：ＮＨＳ与ＣＭＶ感染有关，与ＨＳＶ—Ⅰ、ＨＳＶ—Ⅱ、ＥＢＶ感染关系不大
To investigate the relationship between infant hepatitis syndrome (NHS) and herpes simplex virus (HSV) infection. A pair of primers was designed in the highly homologous DNA polymerase gene of herpesvirus to amplify the four herpesvirus DNAs of HSV-I, HSV-II, EBV and CMV, followed by gel electrophoresis and restriction enzyme BamH Ⅰ or Sma Ⅰ digestion analysis of the amplified products were identified to establish a one-time PCR detection of these four viruses zymography. Sensitivity can be measured 1fgDNA, and the primer only for four herpes virus amplification. The positive rates of CMV, HSV-Ⅰ, HSV-Ⅱ and EBV in urine of 30 children with NHS detected by PCR-zymography were 66.67%, 30%, 3.33% and 6.67% respectively. The positive rate of CMV in 12 neonates with same-age pneumonia cured was 16.7%, which was significantly different from that of NHS (P <0.05). The infection rate of HSV-Ⅰ, HSV-Ⅱ, EBV in NHS and normal children no significant difference. Tip: NHS and CMV infection, and HSV-Ⅰ, HSV-Ⅱ, EBV infection has little to do