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基于L-精氨酸(L-Arginine)对三联吡啶钌(Ru(bpy)32+)在铂电极上的电致发光信号的增敏作用,建立一种测定L-精氨酸的毛细管电泳-电化学发光分离检测新方法。考察了检测电位、吡啶钌浓度、进样高压和进样时间、分离高压、运行缓冲液浓度及pH值等试验参数对L-精氨酸分离度及发光强度的影响,结果表明在最佳试验条件下,检测电位1.15V,Ru(bpy)32+浓度7mmol/L,进样条件8s×10kV,分离高压14kV,运行缓冲液pH8.5,浓度40mmol/L,可在400s内实现对L-精氨酸的分离检测,线性范围为5.74μmol/L～2.53mmol/L(R=0.9995),检出限(S/N=3)为1.38μmol/L。对样品进行测定,5次连续进样的迁移时间和峰高的RSD分别为0.33%和1.96%。本法已成功用于饮料中L-精氨酸的检测。
Based on the sensitization effect of L-Arginine to the electroluminescence signal of Ru (bpy) 32+ on Pt electrode, a capillary electrophoresis- A New Method of Electrochemiluminescence Detection. The influence of test parameters such as the detection potential, ruthenium concentration, injection pressure and injection time, separation pressure, running buffer concentration and pH on L-arginine separation and luminescence intensity were investigated. The results showed that in the best test Under the conditions, the detection potential of 1.15V, Ru (bpy) 32+ concentration of 7mmol / L, injection conditions 8s × 10kV, separation of high pressure 14kV, running buffer pH8.5, concentration 40mmol / L, The detection limit of arginine was 5.74μmol / L ~ 2.53mmol / L (R = 0.9995). The detection limit (S / N = 3) was 1.38μmol / L. The RSDs of migration time and peak height of 5 consecutive injections were 0.33% and 1.96%, respectively. This law has been successfully used in the detection of L-arginine in beverages.