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以金山绣线菊为研究试材,以茎段为外植体,通过使用不同的生长调节剂、不同质量浓度配比对外植体腋芽萌发和增殖影响进行了研究,建立了金山绣线菊无菌培养系;同时,探讨了叶片作为外植体的再生体系的建立。研究结果表明:金山绣线菊的茎段在添加2.0mg·L-16-BA、0.2mg·L-1NAA的MS培养基上腋芽能迅速萌发且长势良好,萌发率为93.3%;叶片在添加2.0mg·L-12,4-D、0.4mg·L-16-BA、0.04mg·L-1IBA的MS培养基上培养20d后产生的愈伤组织,转接到去除2,4-D,添加0.8mg·L-16-BA、0.04mg·L-1NAA的MS培养基上继续培养50d后,不定芽分化率为90%,每个外植体平均再生不定芽数为3.5个;再生植株在添加0.5mg·L-1NAA、0.1%AC(活性炭)的1/2MS培养基上,生根率达到100%,生根苗移植于混合基质中,成活率为86.7%。
In this study, the Tamarindus chinensis was used as the test material and the stem segments were used as explants. The effects of different growth regulators and different mass concentration on the axillary bud germination and proliferation were studied. At the same time, the establishment of regeneration system of leaves as explants was discussed. The results showed that axillary buds could germinate quickly and grew well on the MS medium of 2.0 mg · L-16-BA and 0.2 mg · L-1 NAA in stem segments of Toxoplasma gondii, the germination rate was 93.3% 2.0mg · L-12,4-D, 0.4mg · L-16-BA, 0.04mg · L-1IBA cultured on medium 20d callus generated after 20d, transferred to remove 2,4-D, Addition of 0.8mg · L-16-BA, 0.04mg · L-1NAA on MS medium for 50 days after the continued adventitious bud differentiation rate was 90%, the average number of regenerated adventitious bud per explant 3.5; regenerated plants The rooting rate reached 100% on 1/2 MS medium supplemented with 0.5 mg · L-1 NAA and 0.1% AC (activated carbon). The rooting seedlings were transplanted into the mixed medium with a survival rate of 86.7%.