目的　应用二维超声观测兔VX2肝肿瘤生长及能量多普勒超声造影评价肿瘤血管形成。方法　将VX2 瘤组织块悬液0.3ml (约含107~108 个瘤细胞/ml) 超声引导下注入15只新西兰白兔肝左叶,拔针前针道注入0.2 ml加热的琼脂糖,超声监测肿瘤生长。肿瘤生长至3周时,静脉团注超声造影剂,能量多普勒检测肿瘤血流信号,并通过图像分析技术定量测定肿瘤内彩色血管平均密度(MCVD)。同时二维超声测定肿瘤大小与大体病理的测定结果进行相关性分析。采用免疫组化技术检测肿瘤内微血管密度(MVD)。结果　①二维超声肿瘤表现为等回声、低回声及略高回声,以等回声居多,周围可见声晕;②彩色多普勒和能量多普勒均可检出血流信号;③肿瘤与肝实质无明显边界,可见多个核分裂相;④超声与大体病理下测定肿瘤的最大切面直径有相关关系;⑤能量多普勒超声造影测定肿瘤内MCVD与免疫组化测定的肿瘤内MVD之间有线性相关。结论　超声能有效监测肿瘤生长。能量多普勒超声造影测定肿瘤内MCVD,可用于肿瘤内血管形成的评价。 Objective To evaluate the angiogenesis of tumor by observing the growth of rabbit VX2 liver tumor by using two-dimensional ultrasound and energy Doppler ultrasonography. Methods Fifty New Zealand white rabbits were injected with 0.3 ml of VX2 tumor tissue suspension (about 107-108 tumor cells / ml) under ultrasound guidance into the left hepatic lobe of the New Zealand white rabbits. 0.2 ml of heated agarose was injected into the needle before needle extraction. Tumor growth. Tumor growth to 3 weeks, intravenous injection of ultrasound contrast agent group, the detection of tumor blood flow signal Doppler, and quantitative analysis by image analysis of tumor color vessel average density (MCVD). At the same time, two-dimensional ultrasound was used to determine the correlation between tumor size and gross pathology. Tumor microvessel density (MVD) was detected by immunohistochemistry. Results ① Two-dimensional ultrasound showed equal echogenic, hypoechoic and slightly hypoechoic echoes, with mostly equal echoes and peripheral halo. ② Both color Doppler and energy Doppler were able to detect blood flow signals. ③ Tumor and liver There were no obvious boundaries in real, showing multiple nuclear fission phases. (4) There was a correlation between ultrasonography and the maximum section diameter of tumor in gross pathology. (5) The relationship between intracranial MCVD and intra-tumor MVD in immunohistochemical Linear correlation. Conclusion Ultrasound can effectively monitor tumor growth. The determination of intracisternal MCVD by energy Doppler sonography can be used to evaluate the intravascular tumor formation.