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目的:建立用于测定吴茱萸碱血药浓度的液相色谱-串联质谱联用分析方法,并研究吴茱萸碱在大鼠体内的药代动力学。方法:6只大鼠灌胃给药吴茱萸碱100mg/kg,眼底取血,LC-MS/MS法测定血药浓度,并用DAS药代动力学程序拟合计算药代动力学参数。结果:吴茱萸碱浓度在0.2～50ng/mL内,线性关系良好(r2=0.9997)。提取回收率96.12%～99.46%,日内、日间RSD分别为4.61%～13.51%和5.65%～11.49%。主要药代动力学参数为:Cmax=(5.3±1.5)ng/mL;tmax=(22±8)min;t1/2=(451±176)min。结论:建立的LC-MS/MS方法专属性强,灵敏度高,可用于吴茱萸碱的体内定量分析。
Objective: To establish a liquid chromatography-tandem mass spectrometry method for the determination of evodiamine plasma concentration, and to study the pharmacokinetics of evodiamine in rats. METHODS: Six rats were intragastrically administered with evodiamine 100 mg/kg, and blood was collected from the fundus. Plasma concentrations were determined by LC-MS/MS. The pharmacokinetic parameters were calculated by fitting the DAS pharmacokinetic program. Results: The concentration of evodiamine in the range of 0.2-50 ng/mL showed a good linear relationship (r2=0.9997). The recoveries were 96.12%-99.46%, and the intraday and interday RSD were 4.61%-13.51% and 5.65%-11.49%, respectively. The main pharmacokinetic parameters were: Cmax = (5.3 ± 1.5) ng/mL; tmax = (22 ± 8) min; t1/2 = (451 ± 176) min. Conclusion: The established LC-MS/MS method is highly specific and sensitive, and can be used for in vivo quantitative analysis of evodiamine.