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目的 :确定狗周围神经逆行示踪试验的最佳示踪剂及示踪时间。方法 :用快蓝 (FB)、碘化丙啶 (PI)及辣根过氧化物酶 (HRP)注射于正常狗坐骨神经 ,选择不同时间观察脊髓及相应脊神经背根神经节的神经元。结果 :14天时神经元胞体开始出现 FB,随后荧光逐渐加强和阳性标记细胞逐渐增多 ;而 PI及HRP追踪组 ,仅少量胞体中出现很细的 HRP未能观察到 PI。结论 :狗的周围神经逆行示踪试验最好选择易溶解、激发波长较短的荧光素 ,并在体内存留的时间要长 ,以 FB较为合适。
OBJECTIVE: To determine the best tracer and tracing time for retrograde tracing of peripheral nerves in dogs. Methods: The normal sciatic nerve was injected with fast blue (FB), propidium iodide (PI) and horseradish peroxidase (HRP), and the neurons in the dorsal root ganglion of the spinal cord and corresponding spinal nerves were observed at different time points. Results: At day 14, the FBG began to appear in the somatic cell bodies of the neurons, and then the fluorescence gradually increased and the number of positive labeled cells gradually increased. In the PI and HRP tracing groups, only a small amount of HRP in the small amount of the somatic cells failed to observe the PI. CONCLUSIONS: The retrograde tracing of peripheral nerve of dog is the best choice of easy-dissolving and excitation of fluorescein with shorter wavelength. It takes longer to stay in the body and FB is more suitable.