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目的 了解乙型肝炎病毒e抗原和e抗体双阳性患者血清中病毒前C区基因的变异情况。方法 采用时间分辨荧光免疫分析方法检测乙肝病毒免疫标志物 ,对HBeAg、HBeAb双阳性患者采用聚合酶链反应 (PCR)扩增其血清中HBVDNA前C区基因 ,然后对阳性样品的PCR产物直接标记测序 ,并和Genbank中登录的代表株进行比较分析。结果 1 5例HBeAg、HBeAb双阳性患者中有1 1例HBVDNA阳性 ,序列分析显示所有阳性血清中病毒前C区基因均发生了变异 ,其中有 4例存在A1 896变异。结论 在HBeAg和HBeAb血清学转换过程中 ,均伴有HBV前C区基因变异 ,A1 896变异的产生主要在HBeAb产生过程中或产生以后。
Objective To understand the variation of pre-C gene in serum of hepatitis B e antigen and e antibody double positive patients. Methods Time-resolved immunofluorescence assay was used to detect hepatitis B virus immunological markers. The HBeAg pre-C region gene was amplified by polymerase chain reaction (PCR) in HBeAg and HBeAb double positive patients. Then, the PCR products of positive samples were directly labeled Sequenced, and compared with the representative strains registered in Genbank. Results HBVDNA was positive in 11 cases of HBeAg and HBeAb double positive patients. Sequence analysis showed that all the positive sera had mutations in the pre-C gene, and A1 896 mutation was found in 4 cases. Conclusions During the course of seroconversion of HBeAg and HBeAb, the pre-HBV gene mutation was associated with HBV, and the mutation of A1 896 mainly occurred during or after HBeAb production.