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利用亲和层析和Ｗｅｓｔｅｒｎ－ｂｌｏｔ等分子生物学技术，表达、纯化、鉴定了重组日本血吸虫菲律宾株２６ｋＤａ抗原（ｒＳｊｐ２６ＧＳＴ），并比较了其与日本血吸虫大陆株２６ｋＤａ抗原（ｒＳｊｃ２６ＧＳＴ）基因ｃＤＮＡ序列的异同，分析了其应用前景。分别以质粒ｐＧＥＸ和血吸虫大陆株总ＲＮＡ为模板，经ＰＣＲ或逆转录ＰＣＲ（ＲＴ－ＰＣＲ）扩增出的Ｓｊｐ２６ＧＳＴ和Ｓｊｃ２６ＧＳＴｃＤＮＡ，其片段长度均为６５４ｂｐ。通过双脱氧测序结果显示两者碱基排列顺序相同。用大肠杆菌（Ｅ．ｃｏｌｉ）表达载体在Ｅ．ｃｏｌｉ中高效表达Ｓｊｐ２６ＧＳＴ，ｒＳｊｐ２６ＧＳＴ占菌体总蛋白的２５％。用谷胱甘肽（ＧＳＨ）亲和层析柱一步纯化法，得到纯品Ｓｊｐ２６ＧＳＴ，将其作为靶抗原用Ｗｅｓｔｅｒｎ－ｂｌｏｔ检测血吸虫大陆株感染者和感染小鼠血清及用Ｓｊｃ２６ＧＳＴ免疫的小鼠血清，反应均为阳性。本研究提示Ｓｊｐ２６ＧＳＴ与Ｓｊｃ２６ＧＳＴ在ＤＮＡ序列、氨基酸顺序和抗原性等方面高度同源，Ｓｊｐ２６ＧＳＴ在研制日本血吸虫疫苗方面具有一定应用价值
The 26 kDa antigen (rSjp26GST) of the recombinant Schistosoma japonicum was identified by molecular biology techniques such as affinity chromatography and Western-blot analysis. The rSjp26GST gene was also compared with the cDNA sequence of the 26 kDa antigen (rSjc26GST) of Schistosoma japonicum Similarities and differences, analyzed its application prospects. The fragments of Sjp26GST and Sjc26GST cDNA amplified by PCR or reverse transcription PCR (RT-PCR) were respectively obtained from plasmid pGEX and total RNA of Schistosoma japonicum as template. The fragments were all 654bp in length. By dideoxy sequencing results show that the two base sequence in the same order. The E. coli expression vector was used in E. coli. Sjp26GST was highly expressed in E. coli and rSjp26GST accounted for 25% of total bacterial proteins. Sjp26GST was purified by glutathione (GSH) affinity chromatography column and purified by Sjc26GST. Western blot was used to detect the expression of Sjp26GST in sera of infected and infected mice and serum of mice immunized with Sjc26GST , The reaction was positive. This study suggests that Sjp26GST and Sjc26GST are highly homologous in DNA sequence, amino acid sequence and antigenicity. Sjp26GST has certain value in the development of Schistosoma japonicum vaccine