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目的:探讨大黄附子汤对重症急性胰腺炎(SAP)大鼠炎症反应及胰腺信号转导与转录激活因子(STAT3)表达的影响。方法:45只雄性SD大鼠随机分为3组:假手术组、SAP模型组、大黄附子汤组,每组15只。各组大鼠按造模后处置时间分为3、6、12h3个亚组,每亚组5只。采用5%牛黄胆酸钠胰胆管注射法制备SAP大鼠模型,实验前1h分别灌胃给予生理盐水或大黄附子汤,分别于术后3、6、12h取血,测定血清淀粉酶、肿瘤坏死因子α(TNF-α)、白介素6(IL-6)水平,处死大鼠,取胰腺组织行HE染色检测胰腺病理改变,免疫组化染色检测(STAT3),p-STAT3蛋白表达。结果:大黄附子汤能显著降低SAP大鼠血清淀粉酶、TNF-α、IL-6水平,减轻胰腺炎细胞浸润及组织损伤,降低STAT3,p-STAT3蛋白表达。结论:大黄附子汤通过降低SAP大鼠TNF-α、IL-6水平,减少胰腺STAT3,p-STAT3蛋白表达,减轻胰腺过度的炎症反应,进而发挥保护胰腺作用。
Objective: To investigate the effect of rhubarb and aconite decoction on inflammatory reaction and signal transducers and activators of transcription (STAT3) in severe acute pancreatitis (SAP) rats. Methods: Forty-five male SD rats were randomly divided into three groups: sham operation group, SAP model group and rhubarb and aconite decoction group, 15 rats in each group. The rats in each group were divided into 3, 6, 12h3 subgroups according to the time after modeling, and 5 rats in each subgroup. The rat model of SAP was established by injection of 5% sodium taurocholate into the bile ducts of rats. The rats were administered with normal saline or rhubarb and aconite decoction orally 1 h before the experiment, respectively. Blood samples were taken at 3, 6 and 12 h after operation, serum amylase, tumor necrosis (TNF-α) and interleukin 6 (IL-6) in rats were sacrificed. Rats were sacrificed and their pancreatic tissues were harvested for histopathological changes. The expression of STAT3 and p-STAT3 protein was detected by immunohistochemistry. Results: Rhubarb aconite decoction can significantly reduce serum levels of amylase, TNF-α and IL-6 in SAP rats, alleviate pancreatitis cell infiltration and tissue damage, and decrease STAT3 and p-STAT3 protein expression. Conclusion: Rhubarb aconite decoction can protect the pancreas by decreasing the levels of TNF-α and IL-6 in SAP rats, decreasing the expression of STAT3 and p-STAT3 in pancreas and relieving the excessive inflammatory reaction in pancreas.