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Raji细胞含有众多的EB病毒DNA拷贝数,并且主要以闭锁、环状形式存在。这种EB病毒质粒是与细胞DNA一起复制的。在大多数来源于Burkitt淋巴瘤的细胞株中,EB病毒的合成周期可被各种因子诱导,但化学诱导Raji细胞,只有早期抗原(EA)合成,用P_sHR—1来源的EB病毒重感染则可使Raji细胞中的病毒DNA合成以及病毒外壳抗原(VCA)出现。本实验是用免疫沉淀法结合电泳技术检测化学诱导的和P_sHR—1病毒重感染的Raji细胞中EB病毒专一的多肽的模式。化学药物有正丁酸盐与TPA。重感染实验因病毒滴度不同可分两组。第一组实验30%Raji细胞变
Raji cells contain a large number of Epstein-Barr virus DNA copies and are predominantly in a closed, circular form. This Epstein-Barr virus plasmid replicates with cellular DNA. In most Burkitt lymphoma-derived cell lines, the cycle of Epstein-Barr virus synthesis can be induced by a variety of factors, but chemotactic Raji cells are only synthesized with early antigen (EA), while Epstein-Barr virus-infected Epstein-Barr virus The viral DNA synthesis in Raji cells and the appearance of the viral coat antigen (VCA) can occur. This experiment examined the pattern of Epstein-Barr virus-specific polypeptides in chemically-induced and P_sHR-1-virus-infected Raji cells by immunoprecipitation combined with electrophoresis. The chemical has n-butyrate and TPA. Heavy infection experiment due to different virus titer can be divided into two groups. The first experiment 30% Raji cells change