通过ＰＣＲ扩增ＨＢｓＡｇ基因的Ｓ片段，然后将Ｓ基因与恶性疟原虫杂合４５肽基因连接并克隆到酵母表达质粒ｐＹＥＵｒａ３上。重组质粒被转化到酵母受体菌中。融合基因在酵母中获得表达，表达产物ＨＢｓＡｇ／４５肽，该融合蛋白经亲合层析后被纯化。电镜观察到ＨＢｓＡｇ／４５肽呈颗粒状。Ｄｏｔ┐ＥＬＩＳＡ分析表明恶性疟原虫杂合４５肽抗原可能暴露在颗粒表面 The S fragment of the HBsAg gene was amplified by PCR, and then the S gene was linked to the 45 peptide gene of Plasmodium falciparum and cloned into the yeast expression plasmid pYEUra3. Recombinant plasmids are transformed into yeast recipient bacteria. The fusion gene was expressed in yeast and the product HBsAg / 45 peptide was expressed. The fusion protein was purified by affinity chromatography. Electron microscopy showed that the HBsAg / 45 peptide was granular. Dot ┐ ELISA analysis showed that P. falciparum hybrid 45 peptide antigen may be exposed on the particle surface