目的 :观察反义寡聚核苷酸及其脂质体对角膜基质细胞的通透性。方法 :将荧光标记的 10 0、2 0 μg/ml反义寡聚核苷酸与 5 0、10 μg/ml的反义寡聚核苷酸脂质体加入至兔角膜细胞培养板中 ,分别在 0 5和 4小时 ,荧光显微镜下观察。结果 :0 5小时 ,10 0 μg/ml反义寡聚核苷酸和 5 0、10 μg/ml反义寡聚核苷酸脂质体组细胞内有荧光 ,而 2 0 μg/ml的反义寡聚核苷酸组无荧光 ;4小时 ,所有实验组细胞内都有荧光 ,但 2 0 μg/ml反义寡聚核苷酸组荧光细胞数量少。结论 :游离反义寡聚核苷酸可进入角膜细胞中 ,脂质体包裹有利于反义寡聚核苷酸进入细胞 Objective: To observe the permeability of antisense oligonucleotides and liposomes on corneal stromal cells. METHODS: Fluorescently labeled antisense oligodeoxynucleotides of 10 0 and 20 μg / ml and antisense oligonucleotides of 50 and 10 μg / ml were added into rabbit corneal cell culture plates respectively Fluorescent microscopy was performed at 0 and 4 hours. Results: At 05 hours, the cells were stained with 10 0 μg / ml antisense oligonucleotide and 50, 10 μg / ml antisense oligonucleotide liposomes, while those with 20 μg / ml In the oligodeoxynucleotide group, there was no fluorescence. After 4 hours, all the experimental groups had fluorescence in the cells, but the number of the fluorescent cells in the 20 μg / ml antisense oligonucleotide group was small. CONCLUSIONS: Free antisense oligonucleotides can enter corneal cells and liposome encapsulation facilitates the entry of antisense oligonucleotides into cells