作者曾构建了能表达假狂犬病病毒（PRV)gp50的重组5型腺病毒（Ad-gp50）,它是E1A基因缺陷型病毒,不能在小鼠体内复制,需用高浓度病毒接种,方可诱生低水平保护性抗体。本文报道,用不同配方的油佐剂,能增强免疫动物的抗体应答及对致死量病毒攻击的抵抗作用。 作者用293细胞增殖Ad-gp50,感染细胞经三次冻融破碎后低速离心澄清,用盐水缓冲液将纯化或未经纯化的病毒液稀释成不 The authors have constructed a recombinant adenovirus type 5 (Ad-gp50) that expresses the pseudorabies virus (PRV) gp50, which is an E1A-deficient virus that can not be replicated in mice and is inoculated with a high concentration of virus before being induced Health low level of protective antibodies. This article reports that using different formulations of oil adjuvants can enhance the immune response of immunized animals and the resistance to lethal viral challenge. The authors used 293 cells proliferation Ad-gp50, the infected cells after three freeze-thaw broken clarification by low speed centrifugation, the purified or unpurified virus solution diluted with saline buffer is not