目的研究常见感染菌种及其耐药性变化，指导临床抗生素应用。方法以肉汤培养基或ＸＹＬＢ同步培养基对临床标本进行增菌培养，然后分离、鉴定、进行药敏试验，并对结果统计分析。结果最常见前八位病原菌中机会性致病菌排名提前，细菌耐药率普遍增高，但对氟哌酸、丁胺卡那霉素、头孢哌酮等仍较敏感。结论豫北地区相对老一代较新的抗生素仍有应用价值。为控制细菌感染及新的耐药菌株产生，应严格把握抗生素应用 Objective To study the common infection strains and their drug resistance changes, to guide the clinical application of antibiotics. Methods The broth culture medium or XYLB synchronous culture medium was used to enrich the clinical samples, then isolated, identified and tested for susceptibility. Statistical analysis of the results was also made. Results The most common pathogenic bacteria in the first eight opportunistic pathogenic bacteria ahead of schedule, the general rate of bacterial resistance increased, but norfloxacin, amikacin, cefoperazone is still more sensitive. Conclusion Relatively newer antibiotics in the older generation in Yubei still have applications. In order to control bacterial infections and the emergence of new drug-resistant strains, antibiotics should be strictly controlled