论文部分内容阅读
甘肃马铃薯(2.2kg)经缓冲液提取、硫酸铵分级沉淀、DEAE-纤维素、CM-纤维素离子交换、SephadexG150,Senharose6B葡聚糖凝胶层析纯化、水透析和冻干后制得凝集素,总活力为495120u,比活为6641.89u/mg;经聚丙烯改胺凝胶电泳证明是均一的;氨基酸分析表明富含天冬氨酸、谷氨酸、亮氨酸和赖氨酸。
Gansu potato (2.2kg) was buffer extracted, ammonium sulfate fractionated, DEAE-cellulose, CM-cellulose ion exchange, SephadexG150, Senharose6B dextran gel purification, water dialysis and lyophilization obtained agglutination The total activity was 495120u and the specific activity was 6641.89u / mg. It was proved to be homogeneous by polyacrylamide gel electrophoresis. Amino acid analysis indicated that aspartate, glutamate, leucine and lysine .