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一、免疫扩散试验自1927年开始,就有人用琼脂代替明胶作介质进行免疫扩散试验,获得满意的效果。免疫扩散又称为琼脂扩散。一定浓度的琼脂或琼脂糖制成凝胶后,内部形成多孔的网状结构,孔径很大,可允许分子量在20万以下的大分子物质自由扩散。抗原、抗体的分子量一般都在20万以下,在凝胶中基本上呈自由运动形式,由高浓度区域向低浓度区域扩散时所受阻力也很小。不同的抗原分子由于结构、带电情况、分子量形状、扩散系数等不同,其扩散速
First, the immune spread test Since 1927, some people use agar instead of gelatin as a medium for immunodiffusion test, to obtain satisfactory results. Immune spread is also known as agar diffusion. A certain concentration of agar gel or agarose gel, the internal formation of porous network structure, a large pore size, allowing the molecular weight of 200,000 or less free diffusion of macromolecules. Antigens, antibodies are generally molecular weight below 200,000 in the gel was essentially free form of motion, from the high concentration area to the low concentration area when the diffusion resistance is also very small. Different antigen molecules due to structure, charged situation, molecular weight and shape, diffusion coefficient is different, the diffusion rate