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通过microarray(微列阵芯片)分析,从珠眉海棠盐胁迫cDNA文库中分离得到盐诱导的MzDREBb基因全长cDNA序列,研究苹果属植物DREB转录因子在胁迫中的作用。结果表明:MzDREBb全长1 185bp,开放阅读框共714bp,5′-UTR和3′-UTR的长度分别是121和350bp;MzDREBb编码237个氨基酸,有一个AP2/ERF结构域;系统分类将MzDREBb归入DREB家族的A-5亚组;MzDREBb定位在细胞核中,具有转录激活活性;半定量RT-PCR表明MzDREBb基因受到盐诱导。超表达MzDREBb基因的拟南芥耐盐能力增强。以上结果表明MzDREBb基因在盐胁迫应答中起重要作用。
The full-length cDNA sequence of MzDREBb induced by salt was isolated from the cDNA library of M. cantoniensis by microarray and the effect of DREB transcription factor on stress was studied. The results showed that MzDREBb was 1 185 bp in length and 714 bp in open reading frame. The lengths of 5’-UTR and 3’-UTR were 121 bp and 350 bp, respectively. MzDREBb encoded 237 amino acids with an AP2 / ERF domain. MzDREBb MzDREBb was located in the nucleus and had transcriptional activation activity. Semi-quantitative RT-PCR showed that the MzDREBb gene was induced by salt. Arabidopsis overexpression of MzDREBb gene enhanced salt tolerance. The above results show that the MzDREBb gene plays an important role in salt stress response.