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目的了解无锡市门诊就诊腹泻病例中主要腹泻病毒感染情况。方法连续一年逐月采集无锡市腹泻监测哨点医院门诊腹泻病例的粪便样本,采用实时荧光PCR方法对收集的样本检测诺如病毒(NV)、轮状病毒(RV)、肠道腺病毒(EADV)、札如病毒(SPAV)和星状病毒(ASTV)的核酸,分析病原构成和季节分布变化。结果 291份腹泻样本中,共检出目标病毒核酸阳性79份,总检测阳性率为27.1%,阳性率由高到低依次为NV 15.5%、RV 8.2%、ASTV2.1%、SPAV 1.0%、EADV 0.3%。NV全年各月都有检出,其中GⅡ型占88.9%(40/45),GⅠ型占11.1%(5/45)。这些病毒感染有明显的季节特征,基本表现为冬春季发病高峰的特征。结论无锡地区冬春季节腹泻中病毒性感染值得重视,目前以NV和RV为主,同时,应加强监测优势型别的变化。
Objective To understand the main diarrhea virus infection in outpatient diarrhea cases in Wuxi. Methods The faecal samples from diarrhea clinics of sentinel hospital of sentinel hospital in Wuxi City were collected monthly by month. Real-time fluorescence PCR was used to detect the samples of norovirus (NV), rotavirus (RV), intestinal adenovirus EADV), Sapphire virus (SPAV) and Astrovirus (ASTV) nucleic acids, analysis of pathogenic constitution and seasonal distribution changes. Results Among the 291 diarrhea samples, 79 were positive for the target virus nucleic acid and the total positive rate was 27.1%. The positive rates were NV 15.5%, RV 8.2%, ASTV 2.1% and SPAV 1.0% EADV 0.3%. NV was detected throughout the year, of which G Ⅱ type accounted for 88.9% (40/45), G Ⅰ type accounted for 11.1% (5/45). These virus infections have obvious seasonal characteristics, the basic performance of the peak incidence in winter and spring features. Conclusion The viral infection in diarrhea in winter and spring in Wuxi is worth paying attention to. At present, NV and RV are the main symptoms. At the same time, the changes of dominant type should be monitored.