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目的:观察三七总皂苷对腹膜透析失超滤大鼠模型腹膜形态和超滤功能的影响。方法:实验大鼠随机分为12组:其中高浓度葡萄糖3组(1.5%、2.5%、4.25%);脂多糖(LPS)3组(0.1mg/L、1mg/L、10mg/L);金黄色葡萄糖球菌灭活菌液2组(1011/mL、1015/mL);三七总皂苷干预3组(2.5%葡萄糖溶液+三七总皂苷溶液、1mg/L脂多糖+三七总皂苷溶液、1011/mL金黄色葡萄球菌灭活菌液+三七总皂苷溶液)和正常组。各组大鼠每天腹腔注射相应溶液20mL,20天后做腹膜平衡试验并行腹膜组织病理检查。结果:三七总皂苷可改善高浓度葡萄糖、脂多糖和金黄色葡萄球菌灭菌液引起的腹膜失超滤;高浓度葡萄糖、脂多糖和金黄色葡萄球菌灭菌液均可增加腹膜毛细血管密度,造成毛细血管充血,毛细血管壁增厚,腹膜中炎性细胞浸润。三七总皂苷可以降低腹膜毛细血管密度,明显扩张血管,腹膜毛细血管壁明显增厚、炎性细胞浸润减少。结论:三七总皂苷可改善腹膜超滤功能,其引起的主要腹膜病理改变为腹膜毛细血管密度下降、血管明显扩张,血管壁明显增厚、炎性细胞浸润减少。
Objective: To observe the effects of Panax notoginseng saponins on peritoneal morphology and ultrafiltration function of rat model of peritoneal dialysis. Methods: The experimental rats were randomly divided into 12 groups: high glucose group (1.5%, 2.5%, 4.25%), LPS group (0.1mg / L, 1mg / L and 10mg / L) (1011 / mL, 1015 / mL); Panax notoginseng saponin intervention group (2.5% glucose solution + notoginsenoside solution, 1mg / L lipopolysaccharide + notoginsenoside solution , 1011 / mL Staphylococcus aureus inactivated bacterium + Panax notoginseng saponin solution) and normal group. The rats in each group were injected intraperitoneally with 20 mL of the corresponding solution daily, and the peritoneal balance test was performed 20 days later for peritoneal histopathology. Results: Panax notoginseng saponins could improve the peritoneal loss of ultrafiltration caused by high concentrations of glucose, lipopolysaccharide and Staphylococcus aureus sterilization solution. High concentrations of glucose, lipopolysaccharide and Staphylococcus aureus could increase peritoneal capillary density , Causing capillary congestion, capillary wall thickening, peritoneal inflammatory cell infiltration. Panax notoginseng saponins can reduce peritoneal capillary density, dilated blood vessels, peritoneal capillary wall thickening, decreased inflammatory cell infiltration. Conclusion: Panax notoginseng can improve peritoneal ultrafiltration function. The main peritoneal pathological changes caused by peritoneal capillary degeneration are as follows: the density of peritoneal capillaries is decreased, the blood vessels are obviously dilated, the walls of blood vessels are thickened and the infiltration of inflammatory cells is reduced.