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本文介绍了砷化合物在小鼠多种组织中诱发 SCE 活性的研究情况。对雄性 SWiSS—Webster 小鼠通过吸入与腹腔注射砷化合物进行染毒。仅在吸入浓度为116.5mg/M~3砷酸钙〔Ca_3(AsO_4)_2·3H_2O,(计算浓度)相当于 AS38.61mg/M_3〕时持续染毒10日,每日六小时,在小鼠骨髓细胞、肺泡巨噬细胞以及肝细胞中才观察到 SCE 的轻微升高(骨髓细胞之 SCE、为4.35/细胞,对照组为2.53/细胞)。腹腔注射砷酸钠(Na_2HAsO_4·7H_2O)仅在30mg/kg 染毒组中出现组织细胞间SCE 之差异(骨髓细胞 SCE 为2.51/细胞而肝细胞为5.4/细胞)。与烷化剂相比较,SCE 检查法对砷说来可能是在活体试验中不敏感的方法。试验结果可能表明砷化合物对 DNA 的损伤类型是一种在活体 SCE 检查中无法迅速测出的损伤类型。同时也支持砷可能是一种辅致癌物的假说。
This article describes arsenic compounds in various tissues of mice induced by SCE activity of the research. Male SWiSS-Webster mice were exposed to intraperitoneal injections of arsenic compounds by inhalation. Only in the inhalation concentration of 116.5mg / M ~ 3 calcium arsenate 〔Ca_3 (AsO_4) _2 · 3H_2O, (calculated concentration equivalent to AS38.61mg / M_3]〕 continued exposure to 10 days six hours a day in mice A slight increase in SCE was observed in bone marrow cells, alveolar macrophages, and hepatocytes (SCE of myeloid cells was 4.35 cells / control and 2.53 cells / control). Intraperitoneal injection of sodium arsenate (Na_2HAsO_4 · 7H_2O) showed differences in interstitial SCE only in the 30 mg / kg exposure group (myeloid SCE was 2.51 / cell and hepatocyte was 5.4 / cell). In contrast to alkylating agents, the SCE test may be arsenic-insensitive method in vivo. The test results may indicate that the type of DNA damage caused by arsenic compounds is a type of injury that can not be rapidly detected in living SCE tests. It also supports the hypothesis that arsenic may be a carcinogen.