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目的:探讨大鼠口腔癌变过程中核转录因子κBp65(NF-κBp65)、cyclinD1蛋白的表达及其生物学意义。方法:0.002%4-硝基喹啉-1-氧化物(4NQO)饮水喂养Wistar大鼠9~32周建立大鼠舌癌变模型,用免疫组化SP法检测38例大鼠舌癌变过程不同病理阶段组织中NF-κBP65c、yclinD1蛋白的表达。结果:正常黏膜、轻度异常增生、中度异常增生、重度异常增生、原位癌、鳞癌组织中,NF-κB的阳性率分别是20%、20%、50%、62.5%、50%和83.33%,(鳞癌与正常黏膜组织中NF-κBP65的表达有显著性差异(P<0.05);cyclinD1阳性率分别是20%、60%、62.5%、87.5%、100%和83.33%,重度异常增生、原位癌、鳞癌组织中cyclinD1表达显著高于正常黏膜(P<0.05或P<0.01)。两者的表达均与组织的病理学分级相关(P<0.01),两者的表达在鳞癌阶段具有相关性(P<0.05)。结论:NF-κBp65、cyclinD1蛋白表达与口腔癌发生发展相关,NF-κB通路在口腔黏膜癌变过程起重要作用,NF-κB、cyclinD1可能成为口腔癌变病变生物标志物之一。
Objective: To investigate the expression of nuclear factor κBp65 (NF-κBp65) and cyclinD1 protein in rat oral carcinogenesis and its biological significance. Methods: The rat model of tongue carcinogenesis was established by feeding 0.002% 4-nitroquinoline-1-oxide (4NQO) into Wistar rats for 9 ~ 32 weeks. The pathological changes of tongue in 38 rats were detected by immunohistochemical SP method Expression of NF-κB p65c and yclinD1 in stage tissue. Results: The positive rates of NF-κB in normal mucosa, mild dysplasia, moderate dysplasia, severe dysplasia, carcinoma in situ and squamous cell carcinoma were 20%, 20%, 50%, 62.5%, 50% And 83.33%, respectively (P <0.05). The positive rate of cyclin D1 was 20%, 60%, 62.5%, 87.5%, 100% and 83.33% respectively in squamous cell carcinoma and normal mucosa tissues, The expression of cyclinD1 in severe dysplasia, carcinoma in situ and squamous cell carcinoma was significantly higher than that in normal mucosa (P <0.05 or P <0.01), both of which were correlated with histological grade (P <0.01) (P <0.05) .Conclusion: The expressions of NF-κBp65 and cyclinD1 are correlated with the occurrence and development of oral cancer. NF-κB pathway plays an important role in the carcinogenesis of oral mucosa. The expressions of NF-κB and cyclinD1 may be Oral cancerous lesions one of the biomarkers.