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以拟南芥光合系统Ⅱ PsbS蛋白序列为探针,采用电子克隆与同源克隆结合的方法得到陆地棉光合系统Ⅱ PsbS基因的cDNA序列(GenBank No.ANS53414),进行序列分析,并对其在不同处理下的表达水平进行研究。结果表明:该基因开放阅读框全长837 bp,编码278个氨基酸,蛋白分子量大小为22 kD,为疏水性蛋白,含有两个典型的捕光叶绿素a/b结合蛋白功能域,属于典型的叶绿素a/b结合蛋白家族中的成员。qRT-PCR分析表明,强光、弱光、干旱、高盐、低温胁迫处理后,该基因的表达量整体均呈下降趋势,推测该基因不仅能响应不同光照处理,还能够响应干旱、高盐和低温胁迫,尤其受弱光、高盐和干旱胁迫的诱导表达较为明显。该研究为进一步了解GhPsbS基因对陆地棉高光效育种及抗逆机制的研究提供帮助。
Using the PsbS protein sequence of Arabidopsis thaliana photosystem Ⅱ as probe, the cDNA sequence of PsbS Ⅱ in Upland Cotton (Photosynthetic System Ⅱ) was obtained by the combination of electronic cloning and homologous cloning (GenBank No.ANS53414), and its sequence analysis was carried out. The different levels of expression were studied. The results showed that the open reading frame (ORF) of this gene was 837 bp in length and encoded 278 amino acids. The molecular weight of the open reading frame was 22 kD. It was a hydrophobic protein and contained two typical light-harvesting chlorophyll a / b binding protein domains, belonging to typical chlorophyll a / b members of the binding protein family. qRT-PCR analysis showed that the expression level of this gene showed a downward trend after treated with high light, low light, drought, high salt and low temperature stress. It is speculated that this gene not only responds to different light treatments, but also responds to drought, high salt And low temperature stress, especially induced by weak light, high salt and drought stress. This study will help to further understand the GhPsbS gene on high light efficiency of upland cotton breeding and resistance mechanisms.