应用角蛋白PKK_1、PKK_2、PKK_3、波状蛋白、结蛋白、神经纤维蛋白与胶质纤维酸性蛋白5类7种单克隆抗体研究食管上皮细胞、食管癌、肝癌、肺癌、人胚肺转化细胞、胃癌、星形胶质细胞瘤(BT_(420),BT_(325))、肌细胞、肺细胞及神经纤维细胞等11种培养细胞的中间纤维表达。结果表明,不同细胞具有不同的中间纤维表达的特异性。据此特异性可鉴定胶质细胞、神经细胞、间质细胞、肌细胞与上皮细胞的类型及鉴别正常细胞与肿瘤细胞系的性质与来源。PKK_3还可用于腺癌细胞检测。 To study the effects of seven monoclonal antibodies against keratin PKK_1, PKK_2, PKK_3, vimentin, desmin, nerve fiber and glial fibrillary acidic protein in esophageal epithelial cells, esophageal cancer, liver cancer, lung cancer, human embryo lung transformed cells and gastric cancer , Astroglioma (BT_ (420), BT_ (325)), muscle fibers, lung cells and neurofibrils. The results show that different cells have different specificities of intermediate fiber expression. Based on this specificity, the types of glial cells, nerve cells, interstitial cells, myocytes and epithelial cells can be identified and the nature and origin of normal cells and tumor cell lines can be identified. PKK_3 can also be used for adenocarcinoma cell detection.