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目的探讨缬沙坦对糖尿病大鼠肾脏的保护作用。方法30只SD大鼠随机均分为正常对照组(N组)、糖尿病未干预组、糖尿病大鼠缬沙坦干预组。采用腹腔注射链脲佐菌素(STZ)制备糖尿病动物模型,缬沙坦干预组以缬沙坦10mg/(kg·d)灌胃。8周后通过RT-PCR和Western blot方法,以GAPDH为内参照,检测肾皮质中肾上腺髓质素受体(ADMR)mRNA和蛋白的表达。结果缬沙坦干预组较糖尿病未干预组大鼠体重明显增加(P<0.01),24h尿白蛋白排泄率显著降低,两组糖化血红蛋白及血糖比较无显著性差异;缬沙坦干预组大鼠肾皮质中ADMR mRNA和蛋白的表达显著高于糖尿病未干预组。结论缬纱坦可上调糖尿病大鼠肾皮质中ADMR基因和蛋白的表达;ADMR可能参与了缬沙坦对肾脏的保护作用。
Objective To investigate the protective effect of valsartan on the kidney in diabetic rats. Methods Thirty SD rats were randomly divided into normal control group (N group), untreated diabetic group and valsartan intervention group. Diabetic rats were induced by intraperitoneal injection of streptozotocin (STZ). The valsartan intervention group was given valsartan 10 mg / (kg · d) orally. After 8 weeks, the expression of adrenomedullin receptor (ADMR) mRNA and protein in renal cortex was detected by RT-PCR and Western blot with GAPDH as internal reference. Results The body weight of rats in the valsartan intervention group was significantly higher than that of the diabetic group without intervention (P <0.01), and the urinary albumin excretion rate was significantly lower in the valsartan intervention group. There was no significant difference between the two groups in the glycosylated hemoglobin and the blood glucose level. The expression of ADMR mRNA and protein in renal cortex was significantly higher than those in the non-diabetic diabetic group. Conclusion Valerian can up-regulate the expression of ADMR gene and protein in the renal cortex of diabetic rats. ADMR may be involved in the protective effect of valsartan on the kidney.