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1983年,Hjerten将凝胶引入毛细管中,首次进行了毛细管凝胶电泳(CGE)的研究.至今已使其在分离生物大分子特别是DNA片断时具有其它方法难以达到的效果,其快速、低耗、高效、灵敏的特点已引起生命科学领域的广泛注意.Macek等发现由于低浓度进样时的浓缩效应,长时间的电动进样(25s)并不会使组分区带过分展宽.Paulus等也发现由样品的水溶液进人含7mol/L尿素的凝胶柱中时实际存在着区带锐化效应(或称浓缩效应,等速电泳效应).但文献中均未对这种现象进行详细的研究.本文在自制毛细管凝胶柱上详细考察了进样条件对进样量和区带展宽的影响,为进一步揭示CGE进样机理和规律提供了详实的依据.
Hjerten introduced the gel into a capillary tube for the first time in 1983 and studied capillary electrophoresis (CGE), which has so far made it an unacceptable alternative for the isolation of biological macromolecules, especially DNA fragments, which is fast, low Consumption, efficiency and sensitivity have drawn wide attention in the field of life sciences. Macek et al. Found that prolonged electrokinetic injection (25s) did not excessively broaden the composition zone due to the concentration effect of low concentration injections It was also found that there is a band sharpening effect (or concentration effect, isokinetic electrophoresis effect) actually existing in an aqueous solution of a sample entering a gel column containing 7 mol / L of urea, but no such detail has been reported in the literature The effects of injection conditions on injection volume and zone broadening were investigated in detail on self-made capillary gel columns, providing detailed evidence for further revealing the mechanism and rules of CGE injection.