论文部分内容阅读
实验证明,感染血吸虫的小鼠一次口服吡喹酮300～500mg/kg24小时后,肝内即出现很多死虫,其体表及肠管上皮变性坏死,并有炎细胞侵入虫体。经吡喹酮作用后,两性血吸虫体表组织超微结构的变化主要为:细胞质突起膨大、破裂和形成桥样结构;细胞质内出现含有残余体样及髓鞘样结构的巨大空泡或卵园形小体;杆状、盘状及球状分泌体明显减少,特别是球状分泌体内形成很多空泡,以及体表浆膜有紧密连结变化。体内、体外试验结果表明,经吡喹酮作用后,两性血吸虫体内糖原,碱性磷酸酶及RNA均明显减少或消失;酸性磷酸酶及碱性蛋白质均显著增加,而DNA、酪氨酸、色氨酸、组氨酸,结合α-氨基蛋白质、巯基及酚酶等组成,则无明显变化。 感染血吸虫的小鼠及家兔,于一次口服吡喹酮各为300及60mg/kg后,肉眼观察时,只在少数鼠肝内见有少量的点状凝固性坏死灶,并未见有斑块坏死病变。服药3～4周后,残留的雌虫恢复产卵,但活卵周围的细胞反应很轻,同时,死亡虫卵和变性虫卵周围的纤维结缔组织亦明显减少或消散。此外,服药4～8周后少数兔肝边缘查见血性囊泡,内含被结缔组织包围的活的雄虫。
Experiments show that mice infected with schistosomiasin once oral praziquantel 300 ~ 500mg / kg24 hours, a lot of dead in the liver that the body surface and intestinal epithelial degeneration and necrosis, and inflammatory cells invade the body. After praziquantel treatment, the ultrastructural changes in the surface tissue of Schistosoma japonicum were mainly as follows: the cytoplasm protuberances enlarged, ruptured and formed bridge-like structures; giant vacuoles or oocysts with residual body and myelin-like structures appeared in the cytoplasm Shaped body; rod, disc and spherical secretion was significantly reduced, especially the formation of a lot of spherical secretions vacuoles, and body surface serosa closely linked to changes. In vivo and in vitro test results showed that the glycogen, alkaline phosphatase and RNA of Schistosoma japonicum significantly decreased or disappeared after the praziquantel treatment, while the contents of acid phosphatase and alkaline protein increased significantly, but DNA, tyrosine, Tryptophan, histidine, combined with a-amino protein, mercapto and phenol enzyme composition, no significant change. In mice and rabbits infected with schistosomiasis, after oral administration of 300 and 60 mg / kg each of praziquantel for one week, only a small amount of spot-like coagulative necrosis was seen in the liver of a few mice when observed macroscopically and no plaque was observed Block necrosis. Residual females resumed oviposition after 3 to 4 weeks of treatment, but the cellular responses around the live eggs were very light. Meanwhile, the connective tissue around the dead eggs and detritivores also significantly decreased or dissipated. In addition, 4 to 8 weeks after taking a small number of rabbit liver edge see bloody vesicles, containing living mate surrounded by connective tissue.