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目的探讨年龄因素影响下大鼠大脑皮质细胞中Omi/HtrA2的表达差异及其与神经细胞凋亡的关系。方法采用正常成年及正常老年大鼠大脑皮质组织,经过Caspase-3、8、9、12活性检测和TUNEL法检测凋亡细胞;Western blot法检测Omi/HtrA2蛋白含量变化;Real-timePCR法检测Omi/HtrA2 mRNA含量变化。结果衰老大鼠大脑皮质细胞凋亡明显高于成年大鼠,具体表现为:前者Caspase-3的比活性显著升高,阳性细胞凋亡数显著增高,与后者相比均有统计学差异(P<0.05)。与成年大鼠相比,衰老大鼠大脑皮质Caspase-9的比活性显著升高,二者相比差异有统计学意义(P<0.05);与成年大鼠相比,衰老大鼠大脑皮质Omi/HtrA2蛋白表达水平显著升高,二者相比差异有统计学意义(P<0.01);与成年大鼠相比,衰老大鼠大脑皮质Omi/HtrA2的mRNA水平也显著升高,二者相比差异有统计学意义(P<0.01)。结论衰老大鼠大脑皮质凋亡水平增高,可能与Caspase-9途径活性增高以及Omi/HtrA2的基因和蛋白质表达水平增加有关。
Objective To investigate the difference of Omi / HtrA2 expression in rat cerebral cortex under the influence of age and its relationship with neuronal apoptosis. Methods Normal adult and normal adult rat cortical tissues were used for the detection of apoptotic cells by Caspase-3, 8, 9, 12 activity assay and TUNEL assay. Omi / HtrA2 protein content was detected by Western blot and Real-time PCR / HtrA2 mRNA content changes. Results Apoptosis of cerebral cortex in aged rats was significantly higher than that in adult rats. The specific activity of Caspase-3 was significantly increased and the number of apoptotic cells was significantly higher than that of adult rats P <0.05). Compared with adult rats, the specific activity of Caspase-9 in cerebral cortex of aging rats was significantly increased (P <0.05). Compared with adult rats, the expression of Omi / HtrA2 protein expression was significantly increased, the difference between the two was statistically significant (P <0.01); compared with adult rats, aging rats cerebral cortex Omi / HtrA2 mRNA levels were significantly increased, the two phase The difference was statistically significant (P <0.01). Conclusion The apoptosis of cerebral cortex in aged rats is increased, which may be related to the increased activity of Caspase-9 pathway and the increase of gene and protein expression of Omi / HtrA2.