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目的了解深圳地区艾滋病患者中Ⅰ型人类免疫缺陷病毒(HIV-1)毒株各亚型的感染情况,及其不同亚型对患者疾病进程的影响。方法采用巢式逆转录聚合酶链反应(RTPCR)法对26例艾滋病患者血浆中的HIV-1毒株的env基因片段进行扩增,并对扩增片段进行序列测定和分析,同时检测其CD4+细胞计数与血浆病毒载量。结果基因系统树显示深圳地区26例艾滋病患者的HIV-1毒株env基因序列,B亚型12例,其中1例与欧美B亚型序列十分接近,11例与中国云南B亚型序列十分接近;循环重组亚型AE(CRF01AE)13例,均与泰国AE亚型序列十分接近。比较B、AE亚型感染患者的CD4细胞计数与病毒载量之间的差异无统计学意义。结论深圳地区艾滋病患者中以HIV-1毒株以B亚型和循环重组亚型AE最常见,尚未观察到B、AE亚型对艾滋病进展的影响。
Objective To understand the infection status of HIV-1 strains in HIV-infected patients in Shenzhen and the effects of different subtypes on the disease progression of patients. Methods The env gene fragment of HIV-1 in plasma from 26 AIDS patients was amplified by nested-reverse transcription polymerase chain reaction (RTPCR). The amplified fragment was sequenced and analyzed. Meanwhile, the CD4 + Cell count and plasma viral load. Results The phylogenetic tree showed that the HIV-1 strain env gene was found in 26 AIDS patients in Shenzhen. Twelve cases were identified as B subtypes. One case was closely related to the European subtype B subtype and 11 cases were very close to the Yunnan subtype B subtype ; 13 cases of circulating recombinant subtype AE (CRF01AE), which are very close to the AE subtype of Thailand. There was no significant difference in CD4 cell count and viral load between patients with subtype B and AE subtypes. Conclusion Among AIDS patients in Shenzhen, HIV-1 strains are the most common subtypes of type B and circulating recombinant subtypes. The effects of subtypes B and AE on AIDS progression have not been observed.