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[目的]观察职业铝接触工人认知功能的改变以及血浆中细胞周期依赖性蛋白激酶5(CDK5)与磷酸化tau蛋白的关系。[方法]采用整群抽样法,抽取山西某铝厂418例职业铝接触男性工人作为研究对象。采用简短精神状态量表(MMSE)、数字广度实验(DS)、物体记忆实验(FOM)、简单反应时(SRTT)等问卷测试工人的认知功能。收集工人空腹静脉血样进行实验室分析,石墨炉原子吸收分光光度法检测血浆铝含量;应用ELISA法测定血浆中磷酸化tau-181蛋白(P-tau181)、磷酸化tau-231蛋白(P-tau231)和CDK5蛋白的含量。[结果]根据血铝浓度P25和P75将研究对象分为3组:低血铝组[(64.00±17.78)μg/L]、中血铝组[(132.59±27.45)μg/L]和高血铝组[(244.45±69.86)μg/L]。认知功能测试结果显示,不同血铝水平工人MMSE、DS、FOM、SRTT测试结果不相同(均P<0.05)。蛋白测定结果显示,与低血铝组相比,高血铝组P-tau181、P-tau231浓度增高,中血铝组、高血铝组CDK5浓度增高(P<0.05)。相关分析显示,P-tau181与MMSE、DS、FOM均存在负相关(r=-0.308,-0.285,-0.252;均P<0.05);P-tau231与MMSE、DS存在负相关(r=-0.326,-0.291;均P<0.05),与SRTT存在正相关(r=0.257,P=0.050);P-tau181和P-tau231与CDK5均呈正相关(r=0.327,0.253;均P<0.05)。[结论]职业铝接触导致的认知功能改变与磷酸化tau蛋白有关;作业工人CDK5表达与tau蛋白磷酸化有关,CDK5可能参与铝致tau蛋白磷酸化过程。
[Objective] To observe the changes of cognitive function and the relationship between plasma-dependent cell cycle-dependent protein kinase 5 (CDK5) and phosphorylated tau in professional aluminum exposure workers. [Method] With cluster sampling method, 418 cases of occupational aluminum exposed male workers in an aluminum factory in Shanxi were selected as research objects. The cognitive function of workers was tested using questionnaires such as MMSE, DS, FOM and SRTT. The fasting venous blood samples of workers were collected for laboratory analysis, and the content of plasma aluminum was determined by graphite furnace atomic absorption spectrophotometry. The levels of phosphorylated tau-181 protein (P-tau181), phosphorylated tau-231 protein (P-tau231 ) And CDK5 protein content. [Results] The study groups were divided into three groups according to the blood aluminum concentrations P25 and P75: [(64.00 ± 17.78) μg / L] in the low aluminum group, [(132.59 ± 27.45) μg / L) Aluminum group [(244.45 ± 69.86) μg / L]. The results of cognitive function test showed that MMSE, DS, FOM and SRTT test results were different among workers with different blood levels of aluminum and aluminum (all P <0.05). The results of protein assay showed that the concentrations of P-tau181 and P-tau231 in high blood aluminum group were higher than those in low blood aluminum group, while the concentrations of CDK5 in medium aluminum group and high blood aluminum group were increased (P <0.05). Correlation analysis showed that there was a negative correlation between P-tau181 and MMSE, DS and FOM (r = -0.308, -0.285, -0.252; all P <0.05) , -0.291; all P <0.05), which was positively correlated with SRTT (r = 0.257, P = 0.050). There was a positive correlation between P-tau181 and P-tau231 and CDK5 (r = 0.327,0.253, all P <0.05). [Conclusion] The changes of cognitive function induced by occupational exposure to aluminum are related to the phosphorylation of tau protein. The expression of CDK5 in workers is related to the phosphorylation of tau protein. CDK5 may be involved in the phosphorylation of tau induced by aluminum.