论文部分内容阅读
肝细胞肝癌有极端坏的预后,主要归因于它的高转移性复发.我们曾经报道过对于乙型肝炎相关性肝癌患者,癌周组织的白介素2(IL-2)水平越高就倾向于有更低的肝内转移复发率,而且IL-2的免疫组化显示主要着色于癌周的肝细胞.然而,是否肝细胞能在体外表达IL-2以及其内在机制仍未被揭示.本研究里,比较了有和没有肝炎病史的肝癌患者的癌周组织IL-2表达水平,然后在永生化的成人肝细胞THLE-2里过表达了乙型肝炎病毒x蛋白(HBx),并用过氧化氢处理模拟氧压力微环境.实验证实肝细胞在HBx刺激和低氧压力同时存在下能通过MAP3K7/NF-κB通路表达IL-2.研究结果提示靶向调节微环境氧化压力为预防和治疗乙肝相关性肝癌的转移复发提供了新的方向.
The extremely poor prognosis of hepatocellular carcinoma is largely due to its high metastatic recurrence.We have previously reported that the higher interleukin 2 (IL-2) levels in peri-cancerous tissues tend to be associated with higher levels of IL-2 in patients with hepatitis B-related hepatocellular carcinoma There is a lower rate of recurrence of intrahepatic metastasis, and immunohistochemistry of IL-2 shows predominantly hepatocellular hepatocellular carcinoma.However, whether hepatocytes can express IL-2 in vitro and the underlying mechanisms remain unclear In the study, we compared the expression of IL-2 in peri-cancerous tissues of patients with and without hepatic disease and then overexpressed hepatitis B virus x protein (HBx) in immortalized adult human hepatocytes THLE-2 Hydrogen peroxide treatment mimic oxygen pressure microenvironment.Experimental results confirm that hepatocytes express IL-2 through the MAP3K7 / NF-κB pathway under the simultaneous presence of HBx stimulation and hypoxia stress.The results suggest that targeted regulation of microenvironmental oxidative stress for the prevention and treatment Hepatitis B-related liver cancer metastasis and recurrence provides a new direction.