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目的研究黄芪多糖对连续睡眠剥夺小鼠脾重量、脏器指数和组织结构的影响。方法 78只清洁级雄性小鼠随机分为空白对照组、睡眠剥夺组和黄芪多糖低、中、高剂量组,空白对照组6只,其他每组18只;空白对照组与睡眠剥夺对照组小鼠喂饲蒸馏水,黄芪多糖低、中、高剂量组小鼠分别喂饲添加0.5、1.0和1.5 g/L黄芪多糖的蒸馏水,试验期28 d。平台水环境法进行睡眠剥夺,分别于睡眠剥夺的第24、48和72 h取材;小鼠乙醚呼吸麻醉,立即解剖取脾、称重,4%多聚甲醛PBS液固定,制作石蜡切片,HE染色、显微观测与摄影。结果 (1)睡眠剥夺24 h时,各组间脾重量和脏器指数差异均无统计学意义;睡眠剥夺48 h时,黄芪多糖低、中剂量组脾重量和脏器指数均显著高于两个对照组(P<0.05);睡眠剥夺72 h时,黄芪多糖各剂量组脾重量和脏器指数不同程度高于空白对照组和睡眠剥夺对照组,其中黄芪多糖中、高剂量组脾重量和黄芪多糖各剂量组脾指数显著升高(P<0.05)。(2)睡眠剥夺对照组脾病理组织学变化明显,并随睡眠剥夺时间延长趋于严重;黄芪多糖各剂量组脾组织结构发育较好,与睡眠剥夺时间相同睡眠剥夺对照组比较,脾组织结构表现出较明显的缓解现象。结论不同浓度黄芪多糖对因睡眠剥夺导致小鼠脾和机体的损伤具有缓解和保护作用。
Objective To study the effect of astragalus polysaccharides on spleen weight, organ index and tissue structure in continuous sleep deprivation mice. Methods Seventy-eight clean-grade male mice were randomly divided into blank control group, sleep deprivation group and astragalus polysaccharide low, medium and high dose group, blank control group of 6, the other 18 in each group; blank control group and sleep deprivation control group small Rats were fed with distilled water. Astragalus polysaccharide in low, middle and high dose groups were fed with distilled water containing 0.5, 1.0 and 1.5 g / L APS for 28 days. Plane water environment method for sleep deprivation were taken at 24, 48 and 72 h of sleep deprivation, respectively. The mice were anesthetized with ether breath, immediately dissected and spleen were weighed and fixed in 4% paraformaldehyde PBS solution to make paraffin sections. HE Dyeing, microscopy and photography. Results (1) At 24 h after sleep deprivation, there was no significant difference in spleen weight and organ index between groups. At 48 h after sleep deprivation, the spleen weight and organ index of astragalus polysaccharide group were significantly higher than those of two groups (P <0.05). At 72 h after sleep deprivation, the spleen weight and organ index in astragalus polysaccharide groups were higher than those in blank control group and sleep deprivation control group, and those in astragalus polysaccharide group and high-dose group were significantly higher than those in control group Astragalus polysaccharide in each dose group spleen index was significantly increased (P <0.05). (2) The histopathological changes of spleen in the sleep deprivation control group were obvious, and tended to be serious with the prolongation of sleep deprivation. The spleen tissue structure of astragalus polysaccharide in each dose group was well developed. Compared with sleep deprivation control group, the spleen tissue structure Showed a more obvious relief. Conclusion Different concentrations of Astragalus Polysaccharides can relieve and protect the spleen and body of mice from sleep deprivation.