目的:观察大鼠长期接触低剂量氯化甲基汞(MMC)不同发育阶段仔鼠脑汞含量。方法:雌性Wistar大鼠体重(120±20)g,随机分为3个实验组和对照组各30只。实验组母鼠从妊娠前90天至仔鼠出生后30天连续喂饲含有不同剂量(0.75、1.50和3.00 mg/kg)MMC的普通饲料,对照组给予普通饲料。采用干烧法用F732-V冷原子吸收测汞仪分别测定仔鼠大脑、小脑和海马组织汞含量。结果:在建立MMC所致脑发育损伤动物实验模型过程中,发现实验组母鼠的产子率低于正常鼠,高剂量组尤为明显,生后仔鼠外观无异常表现,不同脑区(大脑、小脑和海马)脑汞含量差异无统计学意义(P>0.05)。各实验组生后不同时间仔鼠脑汞含量明显高于相应对照组(P<0.05),各实验组仔鼠脑汞含量随母鼠接触剂量的增高而有不同程度升高(P<0.05),同一实验组内仔鼠脑汞含量随生后时间的延长逐渐升高,在生后30天达峰值。结论:甲基汞可透过血脑屏障蓄积于仔鼠脑内,且仔鼠脑汞含量与母鼠接触剂量之间存在一定剂量反应关系。 Objective: To observe the content of brain mercury in offspring of rats exposed to low dose of methylmercury chloride (MMC) for a long time. Methods: Female Wistar rats weighing (120 ± 20) g were randomly divided into 3 experimental groups and 30 control groups. The experimental group of rats were fed with normal diet containing different doses of MMC (0.75, 1.50 and 3.00 mg / kg) continuously from 90 days before gestation to 30 days after the birth of the offspring, while the control group were given normal feed. The contents of mercury in brains, cerebellum and hippocampus of offspring were determined by F732-V cold atomic absorption mercury detector with dry method. Results: During the establishment of experimental animal model of brain damage caused by MMC, it was found that the production rate of the mother rats in the experimental group was lower than that in the normal mice especially in the high dose group. The appearances of the offspring were not abnormal. Cerebellum and hippocampus) no significant difference in brain mercury content (P> 0.05). The levels of brain mercury in the offspring of each experimental group were significantly higher than those of the corresponding control group at different time points (P <0.05). The content of brain mercury in each experimental group increased to some extent with the increase of the exposure dose (P <0.05) In the same experimental group, the content of brain mercury in the offspring increased gradually with the extension of time and reached the peak value at 30 days after birth. Conclusion: MeHg can accumulate in the brain of the offspring through the blood-brain barrier, and there is a dose-response relationship between the brain mercury levels in the offspring and the exposure dose of the maternal.