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以日本三陆地区的裙带菜(Undaria pinnatifida)配子体为实验材料,采用双因素完全交叉分组实验,研究了温度(18℃、21℃、24℃、27℃)、光照强度(50μE·m~(-1)·s~(-1)、80μE·m~(-1)·s~(-1)、110μE·m~(-1)·s~(-1)、140μE·m~(-1)·s~(-1))、培养方式(静置培养、磁力搅拌培养、充气悬浮培养)、接种密度(0.1g/L、0.2g/L、0.4g/L、0.6g/L、0.8g/L、1.0g/L)对裙带菜配子体生长的影响。结果显示,光强和温度等环境因素以及两者的交互作用对配子体生长有较大影响。21℃、50μE·m~(-1)·s~(-1)的条件组合是实验中配子体培养的最佳条件。在培养的第5d到第10d之间,裙带菜配子体克隆的细胞增长率最大,可在第10d对配子体进行继代培养,使其保持较高的增长率。培养方式和初始接种密度以及两者的交互作用对配子体生长有较大影响,初始接种密度为0.1~0.2g/L的充气培养条件是配子体培养的最佳条件组合。本实验得出,日本三陆裙带菜雌配子体克隆扩培的最适培养条件为:温度21℃、光强50μE·m~(-1)·s~(-1)、接种密度0.1~0.2g/L,充气悬浮培养。
The effects of temperature (18 ℃, 21 ℃, 24 ℃, 27 ℃), light intensity (50μE · m ~ (-1)) on the population of Undaria pinnatifida gametes in Sanliu region of Japan were studied by means of two- -1) · s -1, 80μE · m -1 s -1, 110μE · m -1 s -1, 140μE · m -1 ) · S ~ (-1)), culture conditions (static culture, magnetic stirring culture and aerated suspension culture), seeding density (0.1g / L, 0.2g / L, 0.4g / L, 0.6g / g / L, 1.0g / L) on the growth of gametophyte. The results showed that environmental factors such as light intensity and temperature, as well as the interaction between the two had a greater impact on gametophyte growth. The combination of conditions of 21 ℃ and 50 μE · m -1 s -1 was the optimal condition for gametophyte culture. Between the 5th day and the 10th day of culture, the clonal growth rate of Undaria pinnatifida gametophyte was the largest, and the gametophyte could be subcultured on the 10th day to maintain a high growth rate. The culture mode, the initial inoculation density and the interaction between the two had a significant effect on the growth of gametophytes. The initial condition of aeration culture with 0.1-0.2 g / L inoculation was the best condition for the gametophyte culture. The results showed that the optimal cultivation conditions for clonal expansion of female gametophyte in Upland plant were as follows: temperature 21 ℃, light intensity 50μE · m -1 s -1, inoculation density 0.1 ~ 0.2g / L, inflatable suspension culture.