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随着生物工程及生命医学及制药工业迅速发展,尤其许多重要的产品被开发,多种生物原物质可通过生物工程获得,但其表达工程菌往往是大肠杆菌.产品易受内毒素污染,迫切需要一种高效能的用于去除内毒素的色谱柱。这是目前生物技术产品中有待解决的问题之一。因此,需要一种能清除内毒素的有效手段,既不破坏原有的有效成份,又能够完全地把内毒素去除。亲和色谱法是利用物质的生物学特性来表达到物质的分离,是去除内毒素的一种期望中较为理想的方法。我们以大孔纤维膜（尼龙66）为介质,以已二胺为间隔臂.以组氨酸或多粘菌素B为配基.制备成亲和层析膜.同时以应用较为灵敏的定量内毒素的检测技术（最低检测限达0.03Eu／ml,最高检测限达0.5Eu／ml）对不同产品制剂内含内毒素去除前后进行定量检测分析。初步进行了自来水含内毒素的清除率,它能达到99.86％,对胸二磷胆碱纳（CytidineDiphosphateCholineCDP-C）内含内毒素的清除率达95.3％,而其药品的有效成分保留车在88％～95.5％。另外,对此膜的一次再生率也能达到99.8％的内毒素清除率。初步结果认为它是在目前方法手段中只为理想的一种清除内毒素的方法。它的最大优点就在于“亲和”对内毒素能较好地予以吸附.对其他有好成份一律不予吸附或吸附量甚微。同时.保留?
With the rapid development of bioengineering and life medicine and pharmaceutical industries, many important bio-products have been developed in particular, and many biogenic bioactive substances can be obtained through bioengineering, but the expression engineering bacteria are often Escherichia coli. There is a need for a highly efficient column for the removal of endotoxins. This is one of the problems to be solved in biotech products. Therefore, there is a need for an effective means of removing endotoxins that can completely remove endotoxin without damaging the original active ingredients. Affinity chromatography is the use of the biological characteristics of the material to express the separation of substances, removal of endotoxin is one of the more desirable method. We use the macroporous membrane (nylon 66) as the medium, the diamine as a spacer arm.His histidine or polymyxin B as ligand.Preparation of affinity chromatography membrane.At the same time, the application of more sensitive quantitative Toxins detection technology (the minimum detection limit of 0.03Eu / ml, the maximum detection limit of 0.5Eu / ml) on different products containing endotoxin removal before and after the quantitative detection analysis. The clearance rate of endotoxin in tap water was initially raised to 99.86%, the clearance rate of endotoxin in Cytidine Diphosphate Choline (CDP-C) reached 95.3%, and the active ingredient of the drug was retained at 88 % ~ 95.5%. In addition, the primary regeneration rate of the membrane reached 99.8% of the endotoxin clearance. The preliminary results suggest that it is an ideal method of removing endotoxins in the current method. Its biggest advantage is that “affinity” to endotoxin can be better to be adsorbed on the other good ingredients will not be adsorbed or adsorbed little. At the same time. Reserved?