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目的:研究昼夜不同时间给予逍遥散对CC l4诱发大鼠慢性肝损伤保护作用的差异及其可能机制。方法:采用40%CC l4皮下注射制备大鼠肝损伤模型,分别于早上8点和晚上8点灌胃给予逍遥散,连续45天,观测大鼠一般行为状态,血清ALT和AST、ALB、TP、A/G、T-B IL、HyP,肝组织匀浆SOD、MDA、GSH-PX、ChE,肝组织病理学变化。结果:与模型组比较,逍遥散能显著改善CC l4诱导大鼠慢性肝损伤行为状态、生化指标、肝中纤维组织增生和变性坏死,使结构紊乱的肝细胞趋于正常;同时,ALT、AST、HyP、T-B IL、SOD、GSH-PX和ChE显示昼夜疗效有显著差别,多数指标显示晚间给药和16.52g/kg剂量组的效果更好。结论:逍遥散慢性肝损伤大鼠具有明显的保肝降酶作用,其疗效与给药时间和剂量密切相关。
Objective: To investigate the protective effect of Xiaoyaosan on CC l4-induced chronic liver injury in rats at different times of day and night and its possible mechanism. Methods: A rat model of hepatic injury was established by subcutaneous injection of 40% CC l4. Xiaoyaosan was orally administered at 8:00 a.m. and 8:00 a.m. for 45 consecutive days. The general behavior, serum ALT and AST, ALB, TP , A / G, TB IL, HyP, liver homogenate SOD, MDA, GSH-PX, ChE, liver histopathological changes. Results: Compared with the model group, Xiaoyao powder can significantly improve the CC l4 induced chronic liver injury in rats behavioral status, biochemical indicators, liver fibrosis and degeneration and necrosis, so that structural disorders of liver cells tend to normal; the same time, ALT, AST HyP, TB IL, SOD, GSH-PX and ChE showed significant differences in day and night efficacy, most of which showed that evening administration and 16.52g / kg dose group were better. Conclusion: Xiaoyaosan chronic liver injury in rats with significant liver and Jiangni role, its efficacy and administration time and dose are closely related.