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Celestine blue(CB)was introduced as a new electroactive indicator in DNA biosensors.The interaction of CB with DNA was investigated by electrochemical and spectroscopic methods.The effect of buffer kind and p H on the electrochemical behavior of CB was studied.The peak currents of CB were linearly related to DNA concentration in the range of 5.0×10~(-9) to 1.0×10~(-7)mol/L.The detection limit of this approach was 4.76×10~(-10) mol/L.Based on spectrometry data a hypochromic effect was observed in UV-Vis spectra of CB with increasing DNA concentration.The results illustrate the possible interaction mode between CB and DNA is electrostatic binding.
Celestine blue (CB) was introduced as a new electroactive indicator in DNA biosensors. The interaction of CB with DNA was investigated by electrochemical and spectroscopic methods. The effect of buffer kind and p H on the electrochemical behavior of CB was studied. The peak currents the detection limit of this approach was 4.76 × 10 ~ (-10) mol / L. The detection limit of this approach was 4.76 × 10 ~ (-10) mol / L. Based on spectrometry data a hypochromic effect was observed in UV-Vis spectra of CB with increasing DNA concentration. These results illustrate the possible interaction mode between CB and DNA is electrostatic binding.