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用ＰＣＲ方法特异性扩增恶性疟原虫云南株（ＰＦＤ－３／ＹＮ）环子孢子蛋白基因片段，经基因序列测定后克隆于ｐＷＲ４５０－１融合蛋白表达载体，并转化大肠杆菌ＴＧ１、ＪＭ１０３及ＪＭ１０９。在不同菌体浓度及不同剂量ＩＰＴＧ诱导下检测ＣＳＰ融合蛋白的表达，结果显示仅ｐＷＲ－ＣＳＰ／ＴＧ１工程菌在菌体浓度ＯＤ５９０值达０．７～０．８时，加入终浓度１ｍｍｏｌ／ＬＩＰＴＧ进行诱导，可表达一８８ｋＤａ的融合蛋白。ｄｏｔ－ＥＬＩＳＡ和Ｗｅｓｔｅｒｎｂｌｏｔ分析表明ＣＳ蛋白表达产物能被抗ＣＳ蛋白重复区单克隆抗体所识别
The gene fragment of circumsporozoite protein of Plasmodium falciparum (PFD-3 / YN) was amplified by PCR and cloned into pWR450-1 fusion protein expression vector and transformed into E. coli TG1, JM103 and JM109 . CSP fusion protein was detected by IPTG induction at different cell concentrations and different concentrations of IPTG. The results showed that only pWR-CSP / TG1 engineered bacteria with the OD590 of 0.7 ~ 0.8 were added with 1mmol / L IPTG After induction, an 88 kDa fusion protein can be expressed. Dot-ELISA and Western blot analysis showed that the CS protein expression product was recognized by the monoclonal antibody against CS protein repeat