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本文用ABC-ELISA法比较研究了HHF和GAH抗原对71例泡球蚴病患者、107例非泡球蚴病患者和86例健康人的诊断价值。结果表明:(1)在以S/N≥2.2为阳性标准时,泡球蚴患者用HHF和GAH抗原的敏感性相同,均为98.59％,假阳性率前者为3.63％,后者为2.07％,经统计学处理两者差别不显著(t=0.92);(2)泡球蚴患者的S/N值在6.0以上者,HHF抗原占67.61％,非常显著地高于GAH的36.62％(t=3.89),棘球蚴患者的HHF抗原占59.75％,也非常显著地高于GAH抗原的6.09％(t=8.91);(3)泡球蚴患者的ABC-ELISA抗体滴度在1∶6400以上者,HHF和GAH抗原各占67.61％和74.61％,两者无显著差别,说明同种和异种抗原均可用于泡球蚴病的诊断;(4)泡球蚴病的ABC—ELISA抗体几何平均滴度用在GAH抗原时为1∶8577.39,为用HHF抗原(1∶7555.04)的1.14倍,而棘球蚴病的抗体几何平均滴度在HHF抗原为1∶2891.39,为GAH抗原(1∶1252.15)的2.31倍,说明在诊断上宜用同种抗原;(5)泡球蚴患者的抗体几何平均滴度比棘球蚴患者高,在用HHF抗原时前者为后者的2.61倍,GAH抗原前者为后者的6.85倍。
ABC-ELISA method was used to compare the diagnostic value of HHF and GAH antigens in 71 cases of alveolar echinococcosis, 107 cases of non-alveolar hydatidosis and 86 cases of healthy people. The results showed that: (1) The sensitivity of HHF and GAH antigens was 98.59% when the S / N≥2.2 was positive, the former was 3.63% and the latter was 2.07% There was no significant difference between the two groups (t = 0.92); (2) The S / N value of metacercariae was 6.0 or more, the proportion of HHF antigen was 67.61%, significantly higher than 36.62% of GAH (t = 3.89), HHF antigen in echinococcosis accounted for 59.75%, which was also significantly higher than that of GAH antigen (6.09%) (t = 8.91). (3) ABC-ELISA antibody titer of metacercariae was above 1:6400 , HHF and GAH antigens each accounted for 67.61% and 74.61%, no significant difference between the two, indicating that allogeneic and heterologous antigens can be used for the diagnosis of alveolar echinococcosis; (4) alveolar hydatidosis ABC-ELISA antibody geometric mean The titer was 1: 8577.39 for the GAH antigen and 1.14 folds for the HHF antigen (1: 7555.04), while the geometric mean titer of the echinococcosis was 1: 2891.39 for the HHF antigen and 1: 1252.15), indicating that the same antigens should be used in the diagnosis. (5) The geometric mean titers of antibodies against metacercariae are higher than those of echinococcosis patients. The former is 2.61 times higher than the latter when using HHF antigens and the GAH The former antigen is the latter 6.85 times.