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本文报道了花生四烯酸代谢对用抗 IgE 刺激后的人嗜碱性白细胞组胺释放(HR)的影响。研究对象为23例患过敏性鼻炎(15)、支气管哮喘(6)和异位性皮炎(2)的异位性患者(其中男13,女10,年龄15～50岁)。11名无异位性的健康人(年龄19～45岁)为对照组。从外周血分离的白细胞(含嗜碱性白细胞)用抗 IgE(山羊抗人 IgE)刺激后上清液中的 HR 和前列腺素 E2(PGE2)的含量,分别用自动荧光分光光度计数和放射免疫试验进行测定。进而测试被试的白细胞在与属环氧合酶
This article reports the effect of arachidonic acid metabolism on histamine release (HR) from human basophils stimulated with anti-IgE. The subjects were 23 patients (13 males, 10 females, and 15 to 50 years of age) with atopic disease in 23 patients with allergic rhinitis (15), bronchial asthma (6) and atopic dermatitis (2). Eleven patients without ectopic health (age 19-45 years old) as the control group. The content of HR and prostaglandin E2 (PGE2) in the supernatant after the white blood cells (including basophils) isolated from peripheral blood were stimulated with anti-IgE (goat anti-human IgE) were measured by autofluorescence spectrophotometry and radioimmunoassay Test to determine. Further test the test of leukocytes with genus cyclooxygenase